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UTSW O'Brien Kidney Center: Animal Models Core

  

Overview of Services

The study of genetically modified mice has greatly expanded our understanding of kidney development and pathophysiology. The UT Southwestern O’Brien Center Animal Models Core provides cutting-edge technology to investigators wishing to generate mouse models for the study of kidney development and/or function.

The Animal Models Core serves three major purposes:

  • To provide the necessary reagents and expertise to mouse geneticists who do not normally study the kidney, to facilitate and encourage their move into this field.
  • To encourage non-mouse geneticists, especially clinical investigators, to venture into the mouse system and to ease this transition.
  • To provide tools to mouse geneticists who do study the kidney, to assist in their studies.

The Core generates and distributes several kidney-specific Cre recombinase and tetracycline-inducible activator and repressor lines to be crossed to floxed or tet responder lines provided by co-investigators. Core personnel assist in generating additional transgenic mice, knockout/in mice, and cell lines, as required by co-investigators. Core personnel also use and train investigators to use various models of organ injury/repair and ex vivo culture. As mentioned, the ultimate purpose of this core is to facilitate research on kidney development and pathophysiology.

Animal Model Process

  • For mouse orders go to Request Services tab
  • Once you initiate the order, fill out the required forms, and click submit.
  • Core staff will review the forms and verify the project meets the NIH requirements.
  • Core staff will enter the information from the forms into the Contracts Office software.
  • Contracts Office will draft the Material Transfer Agreement (MTA) within 2 weeks of the submission date.
  • Once the PI has signed the MTA the Core staff will check the availability of the line requested.
  • If the mice are available, the shipment coordinator will send the health reports to the PI.
  • If the mice are out of stock the Core staff will breed them and notify the PI of an approximate shipment date.
    • UTSW Users: Once the mice are transferred you’ll be billed overnight through PeopleSoft based on the subledger you listed on your order. It will go through the already existing PeopleSoft workflow setup by your division.
    • Non-UTSW Users: You’ll receive an invoice via email. We accept purchase orders, checks, and wire transfers.

 

UT Southwestern George O’Brien Center Mouse models list

 

The UTSW O’Brien Center strives to facilitate research related to the kidney. To accomplish these goals, we maintain a number of “kidney relevant” mouse lines. All strains are maintained in live colonies and can be rapidly provided upon request through iLabs and completion of required forms. Most lines are transferable within UTSW without an MTA. Transfer outside of UTSW may require an MTA with the originating institution. If you have questions on which mouse line is best for your specific applications or the background strain of your line of interest, please contact Tom Carroll (Thomas.carroll@utsouthwestern.edu, 214-648-4187). Please acknowledge the support of the UTSW Obrien Center (P30DK079328) in all publications.

 

 

Name

Mouse Line

Optional information

 

 

 

Loss of function alleles

 

 

Germline nulls

 

 

 

Wnt9b+/-

Null allele of Wnt9b

 

Wnt9b neo/+

Partial loss of function allele of Wnt9b

 

Pard3 +/-

 

 

Vangl2 +/-

 

 

Fat4+/-

Null allele of Fat4

 

Wnt7b exon 3-4 deletion

Null allele of Wnt7b

 

Wnt7b exon 3 deletion

Null allele of Wnt7b

 

Wnt7b exon 1 deletion

Partial loss of function allele of Wnt7b

 

Wnt4+/-

Null allele of Wnt4

 

Smoc2-/-

Null allele of Smoc2

 

Fam132a -/-

Null allele of Fam132a

 

Fam19a5 -/-

Null allele of Fam19a5 (aka Tafa5)

 

Btbd11-/-

Null allele of Btbd11

 

Kl-/-

Null allele of Klotho

Conditional alleles

 

 

 

Wnt9b exon 2 flox

Cre inducible null allele of Wnt9b

 

Wnt7b exon3 flox

Cre inducible null allele of Wnt7b

 

Wnt7b exon 1 flox

Cre inducible partial loss of function allele of Wnt7b

 

Pard3 flox

Cre inducible partial loss of function allele of Par3

 

Lkb1 flox/flox

Cre inducible null allele of Lkb1 (Stk11)

 

Pkd1 flox/flox

Cre inducible null allele of PKD1

 

Srf flox/flox

Cre inducible null allele of Srf

 

Tsc1 flox/flox

Cre inducible null allele of Tsc1

 

Taz flox/flox;Yap flox/flox

Cre inducible null allele of Taz and Yap

 

Lats1/2 flox/flox

Cre inducible null allele of Lats 1 and 2

 

Catnb flox/flox

Cre inducible null allele of beta-catenin

 

cMyc flox/flox

Cre inducible null allele of cMyc

 

Mycn flox/flox

Cre inducible null allele of NMyc

 

cMyc/Nmyc flox/flox

Cre inducible null allele of cMyc and nMyc

Cre activatable cDNAs

 

 

 

Catnb exon3 flox

Cre activatable allele of beta-catenin

 

Rosa-LSL-DTR

Cre activatable allele of Diptheria Toxin receptor

 

Rosa-LSL-DTA

Cre activatable allele of Diptheria Toxin A subunit

 

Rosa LSL Yap5SA

Cre activatable allele of Yap (5 serines mutated to alanine)

 

Rosa-LSL-Wnt1GFP

Cre activatable allele of Wnt1-GFP fusion

 

Cagg-LSL-Wnt9b-IRES-GFP

Cre activatable allele of Wnt9b-IRES-GFP

 

Rosa-lsl-rtTA

Cre activatable allele of reverse Tet activator (Tet-On)

rtTA/tTA inducible cDNAS

 

 

 

Tre-MycN

Tet-responsive NMyc cDNA

 

Tre-Lin28a

Tet-responsive Lin28a cDNA

 

Tre-Lin28b

Tet-responsive Lin28b cDNA

 

Tre-DTA

Tet-responsive Diphtheria toxin A subunit cDNA

 

Tre-H2B-GFP

Tet-responsive Histone 2 B-GFP fusion (nuclear GFP)

 

 

 

Dox inducible lines

KspCad-tTA

Ksp-cadherin promoter driving Tet-activator (Tet-off) cDNA (kidney epithelia driver)

 

KspCad-rtTA

Ksp-cadherin promoter driving reverse Tet-activator (Tet-On) cDNA (kidney epithelia driver)

 

Podocin-rtTA

Podocin promoter driving reverse Tet-activator (Tet-On) cDNA (podocyte driver)

 

Pdgfrb-rtTA

Pdgfrb promoter driving reverse Tet-activator (Tet-On) cDNA (fibroblast/mural cell driver)

Cre lines

 

 

 

Pkhd1Cre

Cre under control of Pkhd1 promoter (late embryonic/postnatal collecting ducts)

 

Wnt5aCre

Cre under control of Wnt5a promoter (posterior mesoderm and renal stroma)

 

Six2Cre-eGFP

Cre-GFP fusion under control of Six2 promoter (nephron progenitor cells and extra-renal cell types)

 

Foxd1Cre-eGFP

Cre-GFP fusion under control of Foxd1 promoter (renal stromal progenitor cells and extra-renal cell types)

 

Sox2Cre

Cre under control of Sox2 promoter (epiblast. Has germline activity in female. Can be used for making null embryos)

 

Rarb2Cre

Cre under control of Rarb2 promoter (intermediate mesoderm and extra-renal cell types)

 

Pax3-Cre

Cre under control of Pax3 promoter (intermediate mesoderm and extra-renal cell types)

 

Hoxb7Cre

Cre under control of Hoxb7 promoter (Wolffian duct and ureteric bud/collecting ducts and extra-renal cell types including posterior neural crest)

 

KspCadherin Cre

Cre under control of cadh-17 promoter (embryonic and adult kidney epithelia. Highest activity in distal segments)

 

T-CreERT2

Tamoxifen inducible Cre under control of Brachyury promoter (mesoderm)

 

Foxd1CreERT2

Tamoxifen inducible Cre under control of Foxd1 promoter (renal stroma and extra-renal cell types)

 

Osr1CreERT2

Tamoxifen inducible Cre under control of Osr1 promoter (intermediate mesoderm and extra-renal cell types)

 

Six2CreERT2

Tamoxifen inducible Cre under control of Six2 promoter (nephron progenitors and extra-renal cell types)

 

Tcf21 CreERT2

Tamoxifen inducible Cre under control of Tcf21 (pod1) promoter (nephron progenitors, stromal progenitors and extra-renal cell types)

 

CaggCreERT2

Tamoxifen inducible Cre under control of Cagg promoter (ubiquitous in embryo and adult)

 

Smoc2CreERT2

Tamoxifen inducible Cre under control of Smoc2 promoter (outer medullary stroma and broad extra-renal stromal activity)

 

KspCreERT2

Tamoxifen inducible Cre under control of cadh-17 promoter (embryonic and adult kidney epithelia. Highest activity in distal segments)

 

Slc34a1CreERT2

Tamoxifen inducible Cre under control of Slc34a1 promoter (S1 and S2 segment of proximal tubule)

 

Gli1CreERT2

Tamoxifen inducible Cre under control of Gli1 promoter (subset of embryonic and adult renal stroma and extra renal cell types)

Reporter lines

 

 

Constitutive reporters

Bat-Gal

Beta-galactosidase transgene under control of optimized Lef/Tcf binding sites. Reports subset of beta-catenin activity

 

Smoc2-tdTomato

tdTomato transgene under control of Smoc2 promoter. Red fluorescence in outer medullary stroma (embryo and adult) and extra renal cell types

 

Mef2c-GFP

GFP transgene under control of Mef2c promoter. Green fluorescence in medullary stroma and extra renal cell types

 

Gucy1a3-GFP

GFP transgene under control of Gucy1a3 promoter. Green fluorescence in medullary stroma and extra renal cell types

 

Cited1-tomato

tdTomato transgene under control of Cited1 promoter. Red fluorescence in nephron progenitor cells   and extra renal cell types

 

Pax8-YFP

GFP transgene under control of Pax8 promoter. Yellow fluorescence in renal vesicles, Mullerian duct and extra renal cell types

 

Tgfb3-GFP

GFP transgene under control of Tgfb promoter. Green fluorescence in cortical stroma, macrophages and extra renal cell types

 

Meis1-GFP

GFP transgene under control of Meis1 promoter. Green fluorescence in all renal stroma and extra renal cell types

Cre inducible reporters

Rosa-LSL- Lacz

Beta-galactosidase transgene under control of Rosa26 promoter upon Cre mediated excision of triple polyadenylation site.

 

Rosa-LSL- myrTom/myrGFP

Myristoylated (membrane) tomato and Myristoylated (membrane) GFP transgenes under control of Rosa26 promoter. All membranes are red until Cre mediated excision of triple Poly A which allows expression of membrane GFP.

 

Rosa-LSL- myrGFP/myrTom

Myristoylated (membrane) GFP and Myristoylated (membrane) Tomato transgenes under control of Rosa26 promoter. All membranes are green until Cre mediated excision of triple Poly A which allows expression of membrane tomato.

 

Rosa-LSL- Tomato

Tomato transgene under control of Rosa26 promoter. Cells fluoresce red upon Cre mediated excision of triple polyadenylation site.

 

Rosa-LSL- YFP

YFP transgene under control of Rosa26 promoter. Cells fluoresce yellow upon Cre mediated excision of triple polyadenylation site.

 

Rosa-Confetti

YFP, CFP and tomato transgenes under control of Rosa26 promoter. Cells stochastically express one of three fluorophores upon Cre mediated excision of triple polyadenylation site.

 

EF1a-LSL-Crb3GFP

Crb3-GFP fusion transgene under control of EF1a promoter. Cells express GFP in apical side of membrane upon Cre mediated excision of triple polyadenylation site. Complete Crb3 cDNA is also expressed. Expression may have gain of function phenotypes.

Doxycycline inducible reporters

Tre-H2bGFP

cDNA encoding Histone 2b GFP fusion protein under control of tet-responsive element. Nuclear GFP can be activated (or inactivated) when combined with tet-repressor or activator lines and doxycycline administration.

 

 

 

 

 

 

 

 

 

 

Administration

Orson W. Moe, M.D.

O'Brien Kidney Center Director

Phone: 214-648-7993

Email: Orson.Moe@UTSouthwestern.edu 

 

Olga Reyes, MS

O'Brien Kidney Center Program Coordinator

Phone: 214-648-7222

E-mail: holga.reyes@utsouthwestern.edu

 

Phone: 214-648-7222

Email: holga.reyes@UTSouthwestern.edu

Leadership

 

Thomas Carroll, Ph.D.

Animal Models Core Co-Director

Phone: 214-648-4187

Email: Thomas.Carroll@UTSouthwestern.edu

 

Christopher Lu, M.D.

Animal Models Core Co-Director

Phone: 214-648-7222

Email: Christopher.Lu@UTSouthwestern.edu

 

Links and Resources

  1. O'Brien Kidney Center Overview
  2. O'Brien Kidney Center Core Services
  3. O'Brien Kidney Center Animal Models Core
  4. O'Brien Kidney Center Physiology Core
  5. O'Brien Kidney Center Cell Biology, Pathology and Imaging Core
  6. O'Brien Kidney Center Clinical and Translational Core

Location and hours of operation

5323 Harry Hines Blvd, Room H5.132

Dallas, TX 75390-8856

Monday - Friday

7AM - 4PM

Contacts

Name Role Phone Email Location
Olga Reyes
Program Coordinator
 
214-648-7222
 
holga.reyes@UTSouthwestern.edu
 
H05.132
 

Price List


Search available services: View: by category alphabetically
Animal Models (2)
Conditional alleles (14)
Cre activatable cDNAs (7)
Cre inducible reporters (7)
Cre lines (19)
Dox inducible lines (4)
Doxycycline inducible reporters (1)
Loss of function alleles Germline nulls (13)
Reporter Lines Constitutive reporters (8)
rtTA/tTA inducible cDNAS (5)

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