UTSW O'Brien Kidney Center: Animal Models Core

  

Overview of Services

The study of genetically modified mice has greatly expanded our understanding of kidney development and pathophysiology. The UT Southwestern O’Brien Center Animal Models Core provides cutting-edge technology to investigators wishing to generate mouse models for the study of kidney development and/or function.

The Animal Models Core serves three major purposes:

  • To provide the necessary reagents and expertise to mouse geneticists who do not normally study the kidney, to facilitate and encourage their move into this field.
  • To encourage non-mouse geneticists, especially clinical investigators, to venture into the mouse system and to ease this transition.
  • To provide tools to mouse geneticists who do study the kidney, to assist in their studies.

The Core generates and distributes several kidney-specific Cre recombinase and tetracycline-inducible activator and repressor lines to be crossed to floxed or tet responder lines provided by co-investigators. Core personnel assist in generating additional transgenic mice, knockout/in mice, and cell lines, as required by co-investigators. Core personnel also use and train investigators to use various models of organ injury/repair and ex vivo culture. As mentioned, the ultimate purpose of this core is to facilitate research on kidney development and pathophysiology.

Animal Model Process

  • For mouse orders go to Request Services tab
  • Once you initiate the order, fill out the required forms, and click submit.
  • Core staff will review the forms and verify the project meets the NIH requirements.
  • Core staff will enter the information from the forms into the Contracts Office software.
  • Contracts Office will draft the Material Transfer Agreement (MTA) within 2 weeks of the submission date.
  • Once the PI has signed the MTA the Core staff will check the availability of the line requested.
  • If the mice are available, the shipment coordinator will send the health reports to the PI.
  • If the mice are out of stock the Core staff will breed them and notify the PI of an approximate shipment date.
    • UTSW Users: Once the mice are transferred you’ll be billed overnight through PeopleSoft based on the subledger you listed on your order. It will go through the already existing PeopleSoft workflow setup by your division.
    • Non-UTSW Users: You’ll receive an invoice via email. We accept purchase orders, checks, and wire transfers.

Animal Core Services

Request Services:
  • b-actin-lsl-Wnt9b
  • CAGG-CreERT2
  • Catnb exon 3 flox
  • Catnb flox
  • Flippase
  • Foxd1-Cre
  • Foxd1CreERT2
  • Hoxb7cre
  • Kif3a mutant and wildtype cell lines
  • Ksp-rtTA
  • Ksp-tTA
  • Ksp/Cre (available through Jackson Laboratories)
  • Ksp/CreERT2
  • Pax8-YFP
  • Podocin rtTA
  • Pkhd1-Cre
  • Rosa Confetti
  • Rosa-Lacz
  • Rosa-LSL-Wnt1
  • Rosa-Wnt1
  • Rosa-YFP
  • RosaTomato
  • Six2-Cre
  • Six2CreERT2
  • SLC12A3-cre
  • TetO-Histone H2B-GFP
  • Tie2Cre
  • Wnt9b flox/flox
  • Wnt9b+/-

Schedule Equipment:

  • Surgical Suite

Training:

  • Embryonic Kidney Organ Cultures
  • Kidney Injury or Repair Model
  • Renal Ischemia-Reperfusion Injury
  • Survival Bleeding Technique
  • Ureteral Obstruction

Administration

Orson W. Moe, M.D.

O'Brien Kidney Center Director

Phone: 214-648-7993

Email: Orson.Moe@UTSouthwestern.edu 

 

Vincy George

O'Brien Kidney Center Program Coordinator

Phone: 214-648-7222

Email: Vincy.George@UTSouthwestern.edu

Leadership

 

Thomas Carroll, Ph.D.

Animal Models Core Co-Director

Phone: 214-648-4187

Email: Thomas.Carroll@UTSouthwestern.edu

 

Christopher Lu, M.D.

Animal Models Core Co-Director

Phone: 214-648-7222

Email: Christopher.Lu@UTSouthwestern.edu

 

Links and Resources

  1. O'Brien Kidney Center Overview
  2. O'Brien Kidney Center Core Services
  3. O'Brien Kidney Center Animal Models Core
  4. O'Brien Kidney Center Physiology Core
  5. O'Brien Kidney Center Cell Biology, Pathology and Imaging Core
  6. O'Brien Kidney Center Clinical and Translational Core

Location and hours of operation

5323 Harry Hines Blvd, Room H5.132

Dallas, TX 75390-8856

Monday - Friday

8AM - 5PM

Contacts

Name Role Phone Email Location
Vincy George
Program Coordinator
 
214-648-7222
 
Vincy.George@UTSouthwestern.edu
 
H05.132
 
Genelyn Vadney
Lab Manager
 
214-648-4189
 
Genelyn.Vadney@UTSouthwestern.edu
 
NA08.124
 
Xinchao Pan
Postdoctoral Researcher
 
214-648-4189
 
Xinchao.Pan@UTSouthwestern.edu
 
NA08.124
 
Keng-Mean Lin
Research Scientist
 
214-648-6785
 
Keng-Mean.Lin@UTSouthwestern.edu
 
H05.120
 
Ebony Flowers
Research Assistant
 
214-648-4189
 
Ebony.Flowers@UTSouthwestern.edu
 
NA08.124
 
Harini Ramalingam
Research Assistant
 
214-648-4189
 
Harini.Ramalingam@UTSouthwestern.edu
 
NA08.124
 
Mary Estes
Technology Transfer Specialist
 
214-648-4305
 
Mary.Estes@UTSouthwestern.edu
 
EB01.360
 
Mansa Melvin
Veterinary Technician
 
214-648-1835
 
Mansa.Melvin@UTSouthwestern.edu
 
ND06.218
 

Price List


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