UTSW O'Brien Kidney Research Core Center: Animal Models Core

Overview of Services

The study of genetically modified mice has greatly expanded our understanding of kidney development and pathophysiology. The U.T. Southwestern O’Brien Kidney Center Animal Models Core provides cutting-edge technology to investigators wishing to generate mouse models for the study of kidney development and/or function.

The Animal Models Core serves three major purposes:

To provide the necessary reagents and expertise to mouse geneticists who do not normally study the kidney, to facilitate and encourage their move into this field.
To encourage non-mouse geneticists, especially clinical investigators, to venture into the mouse system and to ease this transition.
To provide tools to mouse geneticists who do study the kidney, to assist in their studies.

The Core generates and distributes several kidney-specific Cre recombinase and tetracycline-inducible activator and repressor lines to be crossed to floxed or tet responder lines provided by co-investigators. Core personnel assist in generating additional transgenic mice, knockout/in mice, and cell lines, as required by co-investigators. Core personnel also use and train investigators to use various models of organ injury/repair and ex vivo culture. As mentioned, the ultimate purpose of this core is to facilitate research on kidney development and pathophysiology.

All publication require the acknowledgment of the support provided from U.T. Southwestern Medical Center of Dallas, George M. O'Brien Kidney Research Core Center (P30DK079328)

Request Access to Order from our Animal Models Core

1. Top right corner of this webpage click, REGISTER.

- UT Southwestern employee, select, "Register using UTSW credentials." You will be redirected to the UT Southwestern Medical Center Login Page. Log in with your UTSW credentials.

- Non UT Southwestern employee, select, "Register for an iLab account." Enter your institutions e-mail address and follow the steps.

2. You will be directed to an iLab registration page where you will need to select your PI/lab, verify your contact information, etc., complete the registration process.

3. The core will receive an email notification to either approve or deny your request.

4. E-mail your kidney related NIH BioSketch (latest version) to our O'Brien Kidney Center Program Coordinator, Jessica Jurado at jessica.jurado@utsouthwestern.edu.

Access approval is granted within 1 business day after receiveing your NIH BioSketch.

O’Brien Kidney Center Mouse Models List

The UTSW O’Brien Kidney Center strives to facilitate research related to the kidney. To accomplish these goals, we maintain a number of “kidney relevant” mouse lines. All strains are maintained in live colonies and can be rapidly provided upon request through iLabs and completion of required forms. Most lines are transferable within UTSW without an MTA. Transfer outside of UTSW may require an MTA with the originating institution. If you have questions on which mouse line is best for your specific applications or the background strain of your line of interest, please contact Tom Carroll thomas.carroll@utsouthwestern.edu.

Name	Mouse Line	Optional information

Loss of function alleles
Germline nulls
	Wnt9b+/-	Null allele of Wnt9b
	Wnt9b neo/+	Partial loss of function allele of Wnt9b
	Pard3 +/-
	Vangl2 +/-
	Fat4+/-	Null allele of Fat4
	Wnt7b exon 3-4 deletion	Null allele of Wnt7b
	Wnt7b exon 3 deletion	Null allele of Wnt7b
	Wnt7b exon 1 deletion	Partial loss of function allele of Wnt7b
	Wnt4+/-	Null allele of Wnt4
	Smoc2-/-	Null allele of Smoc2
	Fam132a -/-	Null allele of Fam132a
	Fam19a5 -/-	Null allele of Fam19a5 (aka Tafa5)
	Btbd11-/-	Null allele of Btbd11
	Kl-/-	Null allele of Klotho
Conditional alleles
	Wnt9b exon 2 flox	Cre inducible null allele of Wnt9b
	Wnt7b exon3 flox	Cre inducible null allele of Wnt7b
	Wnt7b exon 1 flox	Cre inducible partial loss of function allele of Wnt7b
	Pard3 flox	Cre inducible partial loss of function allele of Par3
	Lkb1 flox/flox	Cre inducible null allele of Lkb1 (Stk11)
	Pkd1 flox/flox	Cre inducible null allele of PKD1
	Srf flox/flox	Cre inducible null allele of Srf
	Tsc1 flox/flox	Cre inducible null allele of Tsc1
	Taz flox/flox;Yap flox/flox	Cre inducible null allele of Taz and Yap
	Lats1/2 flox/flox	Cre inducible null allele of Lats 1 and 2
	Catnb flox/flox	Cre inducible null allele of beta-catenin
	cMyc flox/flox	Cre inducible null allele of cMyc
	Mycn flox/flox	Cre inducible null allele of NMyc
	cMyc/Nmyc flox/flox	Cre inducible null allele of cMyc and nMyc
Cre activatable cDNAs
	Catnb exon3 flox	Cre activatable allele of beta-catenin
	Rosa-LSL-DTR	Cre activatable allele of Diptheria Toxin receptor
	Rosa-LSL-DTA	Cre activatable allele of Diptheria Toxin A subunit
	Rosa LSL Yap5SA	Cre activatable allele of Yap (5 serines mutated to alanine)
	Rosa-LSL-Wnt1GFP	Cre activatable allele of Wnt1-GFP fusion
	Cagg-LSL-Wnt9b-IRES-GFP	Cre activatable allele of Wnt9b-IRES-GFP
	Rosa-lsl-rtTA	Cre activatable allele of reverse Tet activator (Tet-On)
rtTA/tTA inducible cDNAS
	Tre-MycN	Tet-responsive NMyc cDNA
	Tre-Lin28a	Tet-responsive Lin28a cDNA
	Tre-Lin28b	Tet-responsive Lin28b cDNA
	Tre-DTA	Tet-responsive Diphtheria toxin A subunit cDNA
	Tre-H2B-GFP	Tet-responsive Histone 2 B-GFP fusion (nuclear GFP)

Dox inducible lines	KspCad-tTA	Ksp-cadherin promoter driving Tet-activator (Tet-off) cDNA (kidney epithelia driver)
	KspCad-rtTA	Ksp-cadherin promoter driving reverse Tet-activator (Tet-On) cDNA (kidney epithelia driver)
	Podocin-rtTA	Podocin promoter driving reverse Tet-activator (Tet-On) cDNA (podocyte driver)
	Pdgfrb-rtTA	Pdgfrb promoter driving reverse Tet-activator (Tet-On) cDNA (fibroblast/mural cell driver)
Cre lines
	Pkhd1Cre	Cre under control of Pkhd1 promoter (late embryonic/postnatal collecting ducts)
	Wnt5aCre	Cre under control of Wnt5a promoter (posterior mesoderm and renal stroma)
	Six2Cre-eGFP	Cre-GFP fusion under control of Six2 promoter (nephron progenitor cells and extra-renal cell types)
	Foxd1Cre-eGFP	Cre-GFP fusion under control of Foxd1 promoter (renal stromal progenitor cells and extra-renal cell types)
	Sox2Cre	Cre under control of Sox2 promoter (epiblast. Has germline activity in female. Can be used for making null embryos)
	Rarb2Cre	Cre under control of Rarb2 promoter (intermediate mesoderm and extra-renal cell types)
	Pax3-Cre	Cre under control of Pax3 promoter (intermediate mesoderm and extra-renal cell types)
	Hoxb7Cre	Cre under control of Hoxb7 promoter (Wolffian duct and ureteric bud/collecting ducts and extra-renal cell types including posterior neural crest)
	KspCadherin Cre	Cre under control of cadh-17 promoter (embryonic and adult kidney epithelia. Highest activity in distal segments)
	T-CreERT2	Tamoxifen inducible Cre under control of Brachyury promoter (mesoderm)
	Foxd1CreERT2	Tamoxifen inducible Cre under control of Foxd1 promoter (renal stroma and extra-renal cell types)
	Osr1CreERT2	Tamoxifen inducible Cre under control of Osr1 promoter (intermediate mesoderm and extra-renal cell types)
	Six2CreERT2	Tamoxifen inducible Cre under control of Six2 promoter (nephron progenitors and extra-renal cell types)
	Tcf21 CreERT2	Tamoxifen inducible Cre under control of Tcf21 (pod1) promoter (nephron progenitors, stromal progenitors and extra-renal cell types)
	CaggCreERT2	Tamoxifen inducible Cre under control of Cagg promoter (ubiquitous in embryo and adult)
	Smoc2CreERT2	Tamoxifen inducible Cre under control of Smoc2 promoter (outer medullary stroma and broad extra-renal stromal activity)
	KspCreERT2	Tamoxifen inducible Cre under control of cadh-17 promoter (embryonic and adult kidney epithelia. Highest activity in distal segments)
	Slc34a1CreERT2	Tamoxifen inducible Cre under control of Slc34a1 promoter (S1 and S2 segment of proximal tubule)
	Gli1CreERT2	Tamoxifen inducible Cre under control of Gli1 promoter (subset of embryonic and adult renal stroma and extra renal cell types)
Reporter lines
Constitutive reporters	Bat-Gal	Beta-galactosidase transgene under control of optimized Lef/Tcf binding sites. Reports subset of beta-catenin activity
	Smoc2-tdTomato	tdTomato transgene under control of Smoc2 promoter. Red fluorescence in outer medullary stroma (embryo and adult) and extra renal cell types
	Mef2c-GFP	GFP transgene under control of Mef2c promoter. Green fluorescence in medullary stroma and extra renal cell types
	Gucy1a3-GFP	GFP transgene under control of Gucy1a3 promoter. Green fluorescence in medullary stroma and extra renal cell types
	Cited1-tomato	tdTomato transgene under control of Cited1 promoter. Red fluorescence in nephron progenitor cells and extra renal cell types
	Pax8-YFP	GFP transgene under control of Pax8 promoter. Yellow fluorescence in renal vesicles, Mullerian duct and extra renal cell types
	Tgfb3-GFP	GFP transgene under control of Tgfb promoter. Green fluorescence in cortical stroma, macrophages and extra renal cell types
	Meis1-GFP	GFP transgene under control of Meis1 promoter. Green fluorescence in all renal stroma and extra renal cell types
Cre inducible reporters	Rosa-LSL- Lacz	Beta-galactosidase transgene under control of Rosa26 promoter upon Cre mediated excision of triple polyadenylation site.
	Rosa-LSL- myrTom/myrGFP	Myristoylated (membrane) tomato and Myristoylated (membrane) GFP transgenes under control of Rosa26 promoter. All membranes are red until Cre mediated excision of triple Poly A which allows expression of membrane GFP.
	Rosa-LSL- myrGFP/myrTom	Myristoylated (membrane) GFP and Myristoylated (membrane) Tomato transgenes under control of Rosa26 promoter. All membranes are green until Cre mediated excision of triple Poly A which allows expression of membrane tomato.
	Rosa-LSL- Tomato	Tomato transgene under control of Rosa26 promoter. Cells fluoresce red upon Cre mediated excision of triple polyadenylation site.
	Rosa-LSL- YFP	YFP transgene under control of Rosa26 promoter. Cells fluoresce yellow upon Cre mediated excision of triple polyadenylation site.
	Rosa-Confetti	YFP, CFP and tomato transgenes under control of Rosa26 promoter. Cells stochastically express one of three fluorophores upon Cre mediated excision of triple polyadenylation site.
	EF1a-LSL-Crb3GFP	Crb3-GFP fusion transgene under control of EF1a promoter. Cells express GFP in apical side of membrane upon Cre mediated excision of triple polyadenylation site. Complete Crb3 cDNA is also expressed. Expression may have gain of function phenotypes.
Doxycycline inducible reporters	Tre-H2bGFP	cDNA encoding Histone 2b GFP fusion protein under control of tet-responsive element. Nuclear GFP can be activated (or inactivated) when combined with tet-repressor or activator lines and doxycycline administration.

Additional Links and Resources:

Location:

U.T. Southwestern Medical Center of Dallas

O'Brien Kidney Center

5323 Harry Hines Blvd.

Dallas, TX 75390 - 8856

Contacts

Name	Role	Phone	Email
Jessica Jurado, RMA	Program Coordinator	214-648-7222	jessica.jurado@utsouthwestern.edu
Thomas Carroll, Ph.D	Animal Models Core Co-Director	214-648-8147	thomas.carroll@utsouthwestern.edu
Christopher Lu, M.D.	Animal Models Core Co-Director		christopher.lu@utsouthwestern.edu
Orson W. Moe, M.D.	O'Brien Core Center Director		orson.moe@utsouthwestern.edu

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Search available services: View: by category alphabetically

▼ ► Animal Models (2)

▼ ► Conditional alleles (14)

▼ ► Cre activatable cDNAs (7)

▼ ► Cre inducible reporters (7)

▼ ► Cre lines (19)

▼ ► Dox inducible lines (4)

▼ ► Doxycycline inducible reporters (1)

▼ ► Loss of function alleles Germline nulls (13)

▼ ► Reporter Lines Constitutive reporters (8)

▼ ► rtTA/tTA inducible cDNAS (5)