The study of genetically modified mice has greatly expanded our understanding of kidney development and pathophysiology. The U.T. Southwestern O’Brien Kidney Center Animal Models Core provides cutting-edge technology to investigators wishing to generate mouse models for the study of kidney development and/or function.
The Animal Models Core serves three major purposes:
The Core generates and distributes several kidney-specific Cre recombinase and tetracycline-inducible activator and repressor lines to be crossed to floxed or tet responder lines provided by co-investigators. Core personnel assist in generating additional transgenic mice, knockout/in mice, and cell lines, as required by co-investigators. Core personnel also use and train investigators to use various models of organ injury/repair and ex vivo culture. As mentioned, the ultimate purpose of this core is to facilitate research on kidney development and pathophysiology.
All publication require the acknowledgment of the support provided from U.T. Southwestern Medical Center of Dallas, George M. O'Brien Kidney Research Core Center (P30DK079328)
1. Top right corner of this webpage click, REGISTER.
- UT Southwestern employee, select, "Register using UTSW credentials." You will be redirected to the UT Southwestern Medical Center Login Page. Log in with your UTSW credentials.
- Non UT Southwestern employee, select, "Register for an iLab account." Enter your institutions e-mail address and follow the steps.
2. You will be directed to an iLab registration page where you will need to select your PI/lab, verify your contact information, etc., complete the registration process.
3. The core will receive an email notification to either approve or deny your request.
4. E-mail your kidney related NIH BioSketch (latest version) to our O'Brien Kidney Center Program Coordinator, Jessica Jurado at jessica.jurado@utsouthwestern.edu.
Access approval is granted within 1 business day after receiveing your NIH BioSketch.
The UTSW O’Brien Kidney Center strives to facilitate research related to the kidney. To accomplish these goals, we maintain a number of “kidney relevant” mouse lines. All strains are maintained in live colonies and can be rapidly provided upon request through iLabs and completion of required forms. Most lines are transferable within UTSW without an MTA. Transfer outside of UTSW may require an MTA with the originating institution. If you have questions on which mouse line is best for your specific applications or the background strain of your line of interest, please contact Tom Carroll thomas.carroll@utsouthwestern.edu.
Name |
Mouse Line |
Optional information |
|
|
|
Loss of function alleles |
|
|
Germline nulls |
|
|
|
Wnt9b+/- |
Null allele of Wnt9b |
|
Wnt9b neo/+ |
Partial loss of function allele of Wnt9b |
|
Pard3 +/- |
|
|
Vangl2 +/- |
|
|
Fat4+/- |
Null allele of Fat4 |
|
Wnt7b exon 3-4 deletion |
Null allele of Wnt7b |
|
Wnt7b exon 3 deletion |
Null allele of Wnt7b |
|
Wnt7b exon 1 deletion |
Partial loss of function allele of Wnt7b |
|
Wnt4+/- |
Null allele of Wnt4 |
|
Smoc2-/- |
Null allele of Smoc2 |
|
Fam132a -/- |
Null allele of Fam132a |
|
Fam19a5 -/- |
Null allele of Fam19a5 (aka Tafa5) |
|
Btbd11-/- |
Null allele of Btbd11 |
|
Kl-/- |
Null allele of Klotho |
Conditional alleles |
|
|
|
Wnt9b exon 2 flox |
Cre inducible null allele of Wnt9b |
|
Wnt7b exon3 flox |
Cre inducible null allele of Wnt7b |
|
Wnt7b exon 1 flox |
Cre inducible partial loss of function allele of Wnt7b |
|
Pard3 flox |
Cre inducible partial loss of function allele of Par3 |
|
Lkb1 flox/flox |
Cre inducible null allele of Lkb1 (Stk11) |
|
Pkd1 flox/flox |
Cre inducible null allele of PKD1 |
|
Srf flox/flox |
Cre inducible null allele of Srf |
|
Tsc1 flox/flox |
Cre inducible null allele of Tsc1 |
|
Taz flox/flox;Yap flox/flox |
Cre inducible null allele of Taz and Yap |
|
Lats1/2 flox/flox |
Cre inducible null allele of Lats 1 and 2 |
|
Catnb flox/flox |
Cre inducible null allele of beta-catenin |
|
cMyc flox/flox |
Cre inducible null allele of cMyc |
|
Mycn flox/flox |
Cre inducible null allele of NMyc |
|
cMyc/Nmyc flox/flox |
Cre inducible null allele of cMyc and nMyc |
Cre activatable cDNAs |
|
|
|
Catnb exon3 flox |
Cre activatable allele of beta-catenin |
|
Rosa-LSL-DTR |
Cre activatable allele of Diptheria Toxin receptor |
|
Rosa-LSL-DTA |
Cre activatable allele of Diptheria Toxin A subunit |
|
Rosa LSL Yap5SA |
Cre activatable allele of Yap (5 serines mutated to alanine) |
|
Rosa-LSL-Wnt1GFP |
Cre activatable allele of Wnt1-GFP fusion |
|
Cagg-LSL-Wnt9b-IRES-GFP |
Cre activatable allele of Wnt9b-IRES-GFP |
|
Rosa-lsl-rtTA |
Cre activatable allele of reverse Tet activator (Tet-On) |
rtTA/tTA inducible cDNAS |
|
|
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Tre-MycN |
Tet-responsive NMyc cDNA |
|
Tre-Lin28a |
Tet-responsive Lin28a cDNA |
|
Tre-Lin28b |
Tet-responsive Lin28b cDNA |
|
Tre-DTA |
Tet-responsive Diphtheria toxin A subunit cDNA |
|
Tre-H2B-GFP |
Tet-responsive Histone 2 B-GFP fusion (nuclear GFP) |
|
|
|
Dox inducible lines |
KspCad-tTA |
Ksp-cadherin promoter driving Tet-activator (Tet-off) cDNA (kidney epithelia driver) |
|
KspCad-rtTA |
Ksp-cadherin promoter driving reverse Tet-activator (Tet-On) cDNA (kidney epithelia driver) |
|
Podocin-rtTA |
Podocin promoter driving reverse Tet-activator (Tet-On) cDNA (podocyte driver) |
|
Pdgfrb-rtTA |
Pdgfrb promoter driving reverse Tet-activator (Tet-On) cDNA (fibroblast/mural cell driver) |
Cre lines |
|
|
|
Pkhd1Cre |
Cre under control of Pkhd1 promoter (late embryonic/postnatal collecting ducts) |
|
Wnt5aCre |
Cre under control of Wnt5a promoter (posterior mesoderm and renal stroma) |
|
Six2Cre-eGFP |
Cre-GFP fusion under control of Six2 promoter (nephron progenitor cells and extra-renal cell types) |
|
Foxd1Cre-eGFP |
Cre-GFP fusion under control of Foxd1 promoter (renal stromal progenitor cells and extra-renal cell types) |
|
Sox2Cre |
Cre under control of Sox2 promoter (epiblast. Has germline activity in female. Can be used for making null embryos) |
|
Rarb2Cre |
Cre under control of Rarb2 promoter (intermediate mesoderm and extra-renal cell types) |
|
Pax3-Cre |
Cre under control of Pax3 promoter (intermediate mesoderm and extra-renal cell types) |
|
Hoxb7Cre |
Cre under control of Hoxb7 promoter (Wolffian duct and ureteric bud/collecting ducts and extra-renal cell types including posterior neural crest) |
|
KspCadherin Cre |
Cre under control of cadh-17 promoter (embryonic and adult kidney epithelia. Highest activity in distal segments) |
|
T-CreERT2 |
Tamoxifen inducible Cre under control of Brachyury promoter (mesoderm) |
|
Foxd1CreERT2 |
Tamoxifen inducible Cre under control of Foxd1 promoter (renal stroma and extra-renal cell types) |
|
Osr1CreERT2 |
Tamoxifen inducible Cre under control of Osr1 promoter (intermediate mesoderm and extra-renal cell types) |
|
Six2CreERT2 |
Tamoxifen inducible Cre under control of Six2 promoter (nephron progenitors and extra-renal cell types) |
|
Tcf21 CreERT2 |
Tamoxifen inducible Cre under control of Tcf21 (pod1) promoter (nephron progenitors, stromal progenitors and extra-renal cell types) |
|
CaggCreERT2 |
Tamoxifen inducible Cre under control of Cagg promoter (ubiquitous in embryo and adult) |
|
Smoc2CreERT2 |
Tamoxifen inducible Cre under control of Smoc2 promoter (outer medullary stroma and broad extra-renal stromal activity) |
|
KspCreERT2 |
Tamoxifen inducible Cre under control of cadh-17 promoter (embryonic and adult kidney epithelia. Highest activity in distal segments) |
|
Slc34a1CreERT2 |
Tamoxifen inducible Cre under control of Slc34a1 promoter (S1 and S2 segment of proximal tubule) |
|
Gli1CreERT2 |
Tamoxifen inducible Cre under control of Gli1 promoter (subset of embryonic and adult renal stroma and extra renal cell types) |
Reporter lines |
|
|
Constitutive reporters |
Bat-Gal |
Beta-galactosidase transgene under control of optimized Lef/Tcf binding sites. Reports subset of beta-catenin activity |
|
Smoc2-tdTomato |
tdTomato transgene under control of Smoc2 promoter. Red fluorescence in outer medullary stroma (embryo and adult) and extra renal cell types |
|
Mef2c-GFP |
GFP transgene under control of Mef2c promoter. Green fluorescence in medullary stroma and extra renal cell types |
|
Gucy1a3-GFP |
GFP transgene under control of Gucy1a3 promoter. Green fluorescence in medullary stroma and extra renal cell types |
|
Cited1-tomato |
tdTomato transgene under control of Cited1 promoter. Red fluorescence in nephron progenitor cells and extra renal cell types |
|
Pax8-YFP |
GFP transgene under control of Pax8 promoter. Yellow fluorescence in renal vesicles, Mullerian duct and extra renal cell types |
|
Tgfb3-GFP |
GFP transgene under control of Tgfb promoter. Green fluorescence in cortical stroma, macrophages and extra renal cell types |
|
Meis1-GFP |
GFP transgene under control of Meis1 promoter. Green fluorescence in all renal stroma and extra renal cell types |
Cre inducible reporters |
Rosa-LSL- Lacz |
Beta-galactosidase transgene under control of Rosa26 promoter upon Cre mediated excision of triple polyadenylation site. |
|
Rosa-LSL- myrTom/myrGFP |
Myristoylated (membrane) tomato and Myristoylated (membrane) GFP transgenes under control of Rosa26 promoter. All membranes are red until Cre mediated excision of triple Poly A which allows expression of membrane GFP. |
|
Rosa-LSL- myrGFP/myrTom |
Myristoylated (membrane) GFP and Myristoylated (membrane) Tomato transgenes under control of Rosa26 promoter. All membranes are green until Cre mediated excision of triple Poly A which allows expression of membrane tomato. |
|
Rosa-LSL- Tomato |
Tomato transgene under control of Rosa26 promoter. Cells fluoresce red upon Cre mediated excision of triple polyadenylation site. |
|
Rosa-LSL- YFP |
YFP transgene under control of Rosa26 promoter. Cells fluoresce yellow upon Cre mediated excision of triple polyadenylation site. |
|
Rosa-Confetti |
YFP, CFP and tomato transgenes under control of Rosa26 promoter. Cells stochastically express one of three fluorophores upon Cre mediated excision of triple polyadenylation site. |
|
EF1a-LSL-Crb3GFP |
Crb3-GFP fusion transgene under control of EF1a promoter. Cells express GFP in apical side of membrane upon Cre mediated excision of triple polyadenylation site. Complete Crb3 cDNA is also expressed. Expression may have gain of function phenotypes. |
Doxycycline inducible reporters |
Tre-H2bGFP |
cDNA encoding Histone 2b GFP fusion protein under control of tet-responsive element. Nuclear GFP can be activated (or inactivated) when combined with tet-repressor or activator lines and doxycycline administration. |
U.T. Southwestern Medical Center of Dallas
O'Brien Kidney Center
5323 Harry Hines Blvd.
Dallas, TX 75390 - 8856
Name | Role | Phone | Location | |
---|---|---|---|---|
Jessica Jurado, RMA |
Program Coordinator
|
214-648-7222
|
jessica.jurado@utsouthwestern.edu
|
|
Thomas Carroll, Ph.D |
Animal Models Core Co-Director
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214-648-8147
|
thomas.carroll@utsouthwestern.edu
|
|
Christopher Lu, M.D. |
Animal Models Core Co-Director
|
christopher.lu@utsouthwestern.edu
|
||
Orson W. Moe, M.D. |
O'Brien Core Center Director
|
orson.moe@utsouthwestern.edu
|
Request Animal Models |
► Animal Models (2) | |||
Name | Description | Price | |
---|---|---|---|
HoxB7-Venus | Inquire | ||
Podocin rtTA | Inquire | ||
► Conditional alleles (14) | |||
Name | Description | Price | |
Catnb flox/flox |
|
Internal
$100.00
each
External $150.00 each |
|
cMyc flox/flox |
|
Internal
$100.00
each
External $150.00 each |
|
cMyc/Nmyc flox/flox |
|
Internal
$100.00
each
External $150.00 each |
|
Lats1/2 flox/flox |
|
Internal
$100.00
each
External $150.00 each |
|
Lkb1 flox/flox |
|
Internal
$100.00
each
External $150.00 each |
|
Mycn flox/flox |
|
Internal
$100.00
each
External $150.00 each |
|
Pard3 flox |
|
Internal
$100.00
each
External $150.00 each |
|
Pkd1 flox/flox |
|
Internal
$100.00
each
External $150.00 each |
|
Srf flox/flox |
|
Internal
$100.00
each
External $150.00 each |
|
Taz flox/flox;Yap flox/flox |
|
Internal
$100.00
each
External $150.00 each |
|
Tsc1 flox/flox |
|
Internal
$100.00
each
External $150.00 each |
|
Wnt7b exon 1 flox |
Cre inducible partial loss of function allele of Wnt7b |
Internal
$100.00
each
External $150.00 each |
|
Wnt7b exon3 flox |
|
Internal
$100.00
each
External $150.00 each |
|
Wnt9b exon 2 flox |
|
Internal
$100.00
each
External $150.00 each |
|
► Cre activatable cDNAs (7) | |||
Name | Description | Price | |
Cagg-LSL-Wnt9b-IRES-GFP |
|
Internal
$100.00
each
External $150.00 each |
|
Catnb exon3 flox |
|
Internal
$100.00
each
External $150.00 each |
|
Rosa LSL Yap5SA |
|
Internal
$100.00
each
External $150.00 each |
|
Rosa-LSL-DTA |
|
Internal
$100.00
each
External $150.00 each |
|
Rosa-LSL-DTR |
|
Internal
$100.00
each
External $150.00 each |
|
Rosa-lsl-rtTA |
|
Internal
$100.00
each
External $150.00 each |
|
Rosa-LSL-Wnt1GFP |
|
Internal
$100.00
each
External $150.00 each |
|
► Cre inducible reporters (7) | |||
Name | Description | Price | |
EF1a-LSL-Crb3GFP |
|
Internal
$100.00
each
External $150.00 each |
|
Rosa-Confetti |
|
Internal
$100.00
each
External $150.00 each |
|
Rosa-LSL- Lacz |
|
Internal
$100.00
each
External $150.00 each |
|
Rosa-LSL- myrGFP/myrTom |
|
Internal
$100.00
each
External $150.00 each |
|
Rosa-LSL- myrTom/myrGFP |
|
Internal
$100.00
each
External $150.00 each |
|
Rosa-LSL- Tomato |
|
Internal
$100.00
each
External $150.00 each |
|
Rosa-LSL- YFP |
|
Internal
$100.00
each
External $150.00 each |
|
► Cre lines (19) | |||
Name | Description | Price | |
CaggCreERT2 |
|
Internal
$100.00
each
External $150.00 each |
|
Foxd1Cre-eGFP |
|
Internal
$100.00
each
External $150.00 each |
|
Foxd1CreERT2 |
|
Internal
$100.00
each
External $150.00 each |
|
Gli1CreERT2 |
|
Internal
$100.00
each
External $150.00 each |
|
Hoxb7Cre |
|
Internal
$100.00
each
External $150.00 each |
|
KspCadherin Cre |
|
Internal
$100.00
each
External $150.00 each |
|
KspCreERT2 |
|
Internal
$100.00
each
External $150.00 each |
|
Osr1CreERT2 |
|
Internal
$100.00
each
External $150.00 each |
|
Pax3-Cre |
|
Internal
$100.00
each
External $150.00 each |
|
Pkhd1Cre |
|
Internal
$100.00
each
External $150.00 each |
|
Rarb2Cre |
|
Internal
$100.00
each
External $150.00 each |
|
Six2Cre-eGFP |
|
Internal
$100.00
each
External $150.00 each |
|
Six2CreERT2 |
|
Internal
$100.00
each
External $150.00 each |
|
Slc34a1CreERT2 |
|
Internal
$100.00
each
External $150.00 each |
|
Smoc2CreERT2 |
|
Internal
$100.00
each
External $150.00 each |
|
Sox2Cre |
|
Internal
$100.00
each
External $150.00 each |
|
T-CreERT2 |
|
Internal
$100.00
each
External $150.00 each |
|
Tcf21 CreERT2 |
|
Internal
$100.00
each
External $150.00 each |
|
Wnt5aCre |
|
Internal
$100.00
each
External $150.00 each |
|
► Dox inducible lines (4) | |||
Name | Description | Price | |
KspCad-rtTA |
|
Internal
$100.00
each
External $150.00 each |
|
KspCad-tTA |
|
Internal
$100.00
each
External $150.00 each |
|
Pdgfrb-rtTA |
|
Internal
$100.00
each
External $150.00 each |
|
Podocin-rtTA |
|
Internal
$100.00
each
External $150.00 each |
|
► Doxycycline inducible reporters (1) | |||
Name | Description | Price | |
Tre-H2bGFP |
|
Internal
$100.00
each
External $150.00 each |
|
► Loss of function alleles Germline nulls (13) | |||
Name | Description | Price | |
Btbd11-/- |
|
Internal
$100.00
each
External $150.00 each |
|
Fam132a -/- |
|
Internal
$100.00
each
External $150.00 each |
|
Fam19a5 -/- |
|
Internal
$100.00
each
External $150.00 each |
|
Fat4+/- |
|
Internal
$100.00
each
External $150.00 each |
|
Kl-/- |
|
Internal
$100.00
each
External $150.00 each |
|
Pard3 +/- |
Internal
$100.00
each
External $150.00 each |
||
Smoc2-/- |
|
Internal
$100.00
each
External $150.00 each |
|
Vangl2 +/- |
Internal
$100.00
each
External $150.00 each |
||
Wnt4+/- |
|
Internal
$100.00
each
External $150.00 each |
|
Wnt7b exon 1 deletion |
|
Internal
$100.00
each
External $150.00 each |
|
Wnt7b exon 3 deletion |
|
Internal
$100.00
each
External $150.00 each |
|
Wnt7b exon 3-4 deletion |
|
Internal
$100.00
each
External $150.00 each |
|
Wnt9b neo/+ |
|
Internal
$100.00
each
External $150.00 each |
|
► Reporter Lines Constitutive reporters (8) | |||
Name | Description | Price | |
Bat-Gal |
|
Internal
$100.00
each
External $150.00 each |
|
Cited1-tomato |
|
Internal
$100.00
each
External $150.00 each |
|
Gucy1a3-GFP |
|
Internal
$100.00
each
External $150.00 each |
|
Mef2c-GFP |
|
Internal
$100.00
each
External $150.00 each |
|
Meis1-GFP |
|
Internal
$100.00
each
External $150.00 each |
|
Pax8-YFP |
|
Internal
$100.00
each
External $150.00 each |
|
Smoc2-tdTomato |
|
Internal
$100.00
each
External $150.00 each |
|
Tgfb3-GFP |
|
Internal
$100.00
each
External $150.00 each |
|
► rtTA/tTA inducible cDNAS (5) | |||
Name | Description | Price | |
Tre-DTA |
|
Internal
$100.00
each
External $150.00 each |
|
Tre-H2B-GFP |
|
Internal
$100.00
each
External $150.00 each |
|
Tre-Lin28a |
|
Internal
$100.00
each
External $150.00 each |
|
Tre-Lin28b |
|
Internal
$100.00
each
External $150.00 each |
|
Tre-MycN |
|
Internal
$100.00
each
External $150.00 each |