Welcome!
Genetically modified mice are important tools for studying kidney physiology and diseases. However, expertise and equipment for physiological analysis of kidney function in mice are not readily available to many investigators in the renal research community. Furthermore, production of mutant mice has allowed investigators outside the renal community to uncover unexpected renal phenotypes and enter into renal research.
The objective of the Physiology Core is to help participating (established and new) renal investigators with a wide range of physiological techniques to study kidney physiology and diseases at the cellular, organ, and whole animal levels.
The Core will provide free services for certain assays; will provide advice, equipment, and training to assist center investigators’ laboratories to perform other assays; and will arrange collaborations with core directors on certain techniques that require extensive effort and advanced training.
This assay is provided by the O'Brien Center as a service that incurs non-profit cost-recovery charges. In contrast to the commercial ELISA, we use an immunoprecipitation-immunblot assay using synthetic anti-Klotho antibodies that reliably detects full-length Klotho in sera and urine from human, rat, and mouse.
Compared to the commercial assay, the drawback of our IP-IB assay that it requires higher sample volume and cannot achieve high throughput mode, because of the laborious nature of the assay. However, the main advantage is the highly specific and sensitive reagents and known performance of inter-assay variation of 7 percent and intra-assay variation of < 2 percent.
| Name | Role | Phone | Location | |
|---|---|---|---|---|
| Jessica Lucas |
Capillary Electrophoresis
|
214-648-0240
|
Jessica.Lucas@UTSouthwestern.edu
|
H5.102
|
| Susan Legan |
Blood Gas Machine
|
214-648-3438
|
Susan.Legan@UTSouthwestern.edu
|
G3.250
|
| Johanne Pastor |
Klotho Assay
|
214-648-1177
|
Johanne.Pastor@UTSouthwestern.edu
|
G4.210
|
| Orson W. Moe, M.D. |
O'Brien Kidney Center Director
|
214-648-2754
|
orson.moe@utsouthwestern.edu
|
|
| Michel Baum, M.D. |
Physiology Core Director
|
214-648-3438
|
michel.baum@utsouthwestern.edu
|
|
| Matthias Wolf, M.D. |
Physiology Core Co-Director
|
214-648-3438
|
matthias.wolf@utsouthwestern.edu
|
| Services |
| ► Services (16) | |||
| Name | Description | Price | |
|---|---|---|---|
| Magnesium in tissue via atomic absorption |
Internal
$13.00
each
External $15.00 each Commercial/Industry $30.00 each |
||
| Magnesium in urine, serum/plasma via atomic absorption |
Internal
$10.00
each
External $11.00 each Commercial/Industry $22.00 each |
||
| Urea Measurement Assay |
Quantitative Colorimetric Urea Determination in serum, plasma and urine. The assay requires 30 microliters and the cost is $10.00 per sample. |
Internal
$10.00
each
UT System/In Network $10.00 each External $10.00 each Commercial/Industry $20.00 each UTSW Generic $10.00 each |
|
| Klotho IP-IB Assay (plasma) |
Internal
$15.00
each
External $17.00 each Commercial/Industry $34.00 each |
||
| Klotho IP-IB Assay (plasma, less than 50 samples) |
Internal
$17.00
each
External $22.00 each |
||
| Klotho IP-IB Assay (serum) |
Internal
$12.00
each
External $14.00 each Commercial/Industry $28.00 each |
||
| Klotho IP-IB Assay (serum, less than 50 samples) |
Internal
$11.00
each
External $16.00 each Commercial/Industry $32.00 each |
||
| Klotho IP-IB Assay (urine) |
Internal
$15.00
each
External $17.00 each Commercial/Industry $34.00 each |
||
| Klotho IP-IB Assay (creatinine) |
|
Internal
$2.00
each
External $6.00 each Commercial/Industry $12.00 each |
|
| Measurement of Citrate by Capillary Electrophoresis (serum) |
|
Internal
$11.00
each
External $18.00 each Commercial/Industry $36.00 each |
|
| Measurement of Citrate by Capillary Electrophoresis (urine) |
|
Internal
$11.00
each
External $18.00 each Commercial/Industry $36.00 each |
|
| Measurement of Creatinine by Capillary Electrophoresis (serum) |
Internal
$6.00
each
External $10.00 each Commercial/Industry $20.00 each |
||
| Measurement of Creatinine by Capillary Electrophoresis (serum, expedited) |
|
Internal
$12.00
each
External $20.00 each Commercial/Industry $40.00 each |
|
| Measurement of Creatinine by Capillary Electrophoresis (urine) |
Internal
$6.00
each
External $10.00 each Commercial/Industry $20.00 each |
||
| Measurement of Creatinine by Capillary Electrophoresis (urine, expedited) |
|
Internal
$12.00
each
External $20.00 each Commercial/Industry $40.00 each |
|
| Microanalysis of serum and urine electrolytes |
|
Internal
$15.00
each
External $25.00 each Commercial/Industry $50.00 each |
|
| ► Collaboration (6) | |||
| Name | Description | Price | |
| Hydroxycitrate in Media |
By Collaboration Only Contact Jessica Lucas (jessica.lucas@utsouthwestern.edu) for more information. |
Inquire | |
| Hydroxycitrate in Serum |
By Collaboration Only Contact Jessica Lucas (jessica.lucas@utsouthwestern.edu) for more information. |
Inquire | |
| Hydroxycitrate in Urine |
By Collabortation Only Please contact Jessica Lucas (jessica.lucas@utsouthwestern.edu) for more information. |
Inquire | |
| Electrophysiological studies of ion channels by patch-clamp recordings |
|
Inquire | |
| Microdissection of tubules for quantification of proteins/gene expression |
|
Inquire | |
| Microperfusion, dissection of individual tubules for real time PCR, western blot, and immunohistochemistry |
|
Inquire | |
| ► Resources (4) | |||
| Name | Description | Price | |
| Blood-pressure measurement by tail-cuff method (mice) |
|
Internal
$0.00
each
External $0.00 each |
|
| Blood-pressure measurement by tail-cuff method (rats) |
|
Internal
$0.00
each
External $0.00 each |
|
| Clearance studies in live animals by metabolic cages |
Metabolic cages should be would be cleaned thoroughly before returning:
|
Internal
$0.00
each
External $0.00 each |
|
| Measurement of serum and urine chemistry and blood gas |
|
Internal
$20.00
each
External $40.00 each Commercial/Industry $80.00 each |
|
