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UTSW O'Brien Kidney Research Core Center: Physiology Core

Overview of Services

Genetically modified mice are important tools for studying kidney physiology and diseases. However, expertise and equipment for physiological analysis of kidney function in mice are not readily available to many investigators in the renal research community. Furthermore, production of mutant mice has allowed investigators outside the renal community to uncover unexpected renal phenotypes and enter into renal research.

The objective of the Physiology Core is to help participating (established and new) renal investigators with a wide range of physiological techniques to study kidney physiology and diseases at the cellular, organ, and whole animal levels.

The Core will provide free services for certain assays; will provide advice, equipment, and training to assist center investigators’ laboratories to perform other assays; and will arrange collaborations with core directors on certain techniques that require extensive effort and advanced training. 

Klotho IP-IB Assay

This assay is provided by the O'Brien Center as a service that incurs non-profit cost-recovery charges. In contrast to the commercial ELISA, we use an immunoprecipitation-immunblot assay using synthetic anti-Klotho antibodies that reliably detects full-length Klotho in sera and urine from human, rat, and mouse.

Compared to the commercial assay, the drawback of our IP-IB assay that it requires higher sample volume and cannot achieve high throughput mode, because of the laborious nature of the assay. However, the main advantage is the highly specific and sensitive reagents and known performance of inter-assay variation of 7 percent and intra-assay variation of < 2 percent.

 

Request Services:

  • Magnesium in urine, serum/plasma, and tissue via atomic absorption
  • Measurement of serum and or urine creatinine by capillary electrophoresis
  • Measurement of serum and urine citrate by capillary electrophoresis (in development)
  • Microanalysis of serum and urine electrolytes via flame photometry, Atomic absorption or ion-selective electrode
  • Klotho IP-IB Assay

 

Schedule Equipment:

  • Blood-pressure measurement in mice and rats by tail-cuff method (training provided)
  • Measurement of serum and urine chemistry and blood gas (training provided)
  • Clearance studies in live animals by metabolic cages

 

Collaboration:

  • Microdissection of tubules for quantitation of proteins/gene expression
  • Electrophysiological studies of ion channels by patch-clamp recordings
  • Microperfusion, dissection of individual tubules for real time PCR, western blot, and immunohistochemistry

 

 

Links and Resources

  1. O'Brien Kidney Center Overview
  2. O'Brien Kidney Center Core Services
  3. O'Brien Kidney Center Animal Models Core
  4. O'Brien Kidney Center Physiology Core
  5. O'Brien Kidney Center Cell Biology and Imaging Core
  6. O'Brien Kidney Center Clinical and Translational Core

Contacts

Name Role Phone Email Location
Jessica Jurado, RMA
O'Brien Kidney Center Program Coordinator
 
214-648-7222
 
jessica.jurado@UTSouthwestern.edu
 

 
Jessica Lucas
Capillary Electrophoresis
 
214-648-0240
 
Jessica.Lucas@UTSouthwestern.edu
 
H5.102
 
Susan Legan
Blood Gas Machine
 
214-648-3438
 
Susan.Legan@UTSouthwestern.edu
 
G3.250
 
Johanne Pastor
Klotho Assay
 
214-648-1177
 
Johanne.Pastor@UTSouthwestern.edu
 
G4.210
 
Orson W. Moe, M.D.
O'Brien Kidney Center Director
 
214-648-2754
 
orson.moe@utsouthwestern.edu
 

 
Michel Baum, M.D.
Physiology Core Director
 
214-648-3438
 
michel.baum@utsouthwestern.edu
 

 
Matthias Wolf, M.D.
Physiology Core Co-Director
 
214-648-3438
 
matthias.wolf@utsouthwestern.edu
 

 

Services


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