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UTSW O'Brien Kidney Research Core Center: Cell Biology, Pathology and Imaging Core

  

Overview of Services

 

The objectives of the Cell Biology, Pathology and Imaging Core are to provide high quality histopathologic and scientific support for research projects by localizing proteins and/or mRNAs in tissues and cultured cells, and to offer pathology support. The Core maintains a library of wild-type murine embryos and adult tissues embedded in paraffin for use by O'Brien Center investigators. The lab also maintains bright-field, dark-field, phase-contrast, epifluorescence, and confocal microscopes.

Pathology Services

  • Processing of tissues for routine paraffin embedding
  • Detailed paraffin and cryo-microtomy
  • Standard histologic stains and special stains when required
  • Immunohistochemistry and in situ hybridization
  • Kidney pathology
  • Single tubule isolation and immunocytochemistry
  • Laser microdissection

 

Schedule Equipment:

  • Zeiss LSM 510 Meta Confocal Microscope
    • Training provided for the following
      • Image Fixed Cells
      • Image Live Cells
      • Zen Software
  • Imaging of rodent kidney and heart including functional MRI
    • Consultation with Dr. Masaya Takahashi
    • Investigators are responsible for time reserved on the magnet.
      • Apparent diffusion coefficient (fibrosis)
      • Arterial spin labeling (blood flow)
      • BOLD (oxygenation) Na MRS 

 

Links and Resources

  1. O'Brien Kidney Center Overview
  2. O'Brien Kidney Center Core Services
  3. O'Brien Kidney Center Animal Models Core
  4. O'Brien Kidney Center Physiology Core
  5. O'Brien Kidney Center Cell Biology and Imaging Core
  6. O'Brien Kidney Center Clinical and Translational Core
  7. MRI Tools and References

 

Contacts

Name Role Phone Email Location
Jessica Jurado, RMA
O'Brien Kidney Center Program Coordinator
 
214-648-7222
 
jessica.jurado@UTSouthwestern.edu
 

 
Leslye Grant
Confocal Microscope Trainer
 
214-648-3496
 
Leslye.Grant@UTSouthwestern.edu
 
G5.100
 
Orson W. Moe, M.D.
O'Brien Kidney Center Director
 
214-648-2754
 
orson.moe@utsouthwestern.edu
 

 
Denise Marciano, M.D., Ph.D.
Cell Biology and Imaging Core Co-Director
 
214-648-3966
 
denise.marciano@utsouthwestern.edu
 

 
Bret Evers, Ph.D.
PA-Anatomical Pathology
 
214-648-2148
 
bret.evers@utsouthwestern.edu
 

 

Price List


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Histology Services (30)
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