UTSW O'Brien Kidney Research Core Center: Cell Biology, Pathology and Imaging Core

Overview of Services

The objectives of the Cell Biology, Pathology and Imaging Core are to provide high quality histopathologic and scientific support for research projects by localizing proteins and/or mRNAs in tissues and cultured cells, and to offer pathology support. The Core maintains a library of wild-type murine embryos and adult tissues embedded in paraffin for use by O'Brien Center investigators. The lab also maintains bright-field, dark-field, phase-contrast, epifluorescence, and confocal microscopes.

Pathology Services

• Processing of tissues for routine paraffin embedding
• Detailed paraffin and cryo-microtomy
• Standard histologic stains and special stains when required
• Immunohistochemistry and in situ hybridization
• Kidney pathology
• Single tubule isolation and immunocytochemistry
• Laser microdissection

Schedule Equipment:

• Zeiss LSM 510 Meta Confocal Microscope
  • Training provided for the following
    • Image Fixed Cells
    • Image Live Cells
    • Zen Software
• Imaging of rodent kidney and heart including functional MRI
  • Consultation with Dr. Masaya Takahashi
  • Investigators are responsible for time reserved on the magnet.
    • Apparent diffusion coefficient (fibrosis)
    • Arterial spin labeling (blood flow)
    • BOLD (oxygenation) Na MRS 

Links and Resources

1. O'Brien Kidney Center Overview
2. O'Brien Kidney Center Core Services
3. O'Brien Kidney Center Animal Models Core
4. O'Brien Kidney Center Physiology Core
5. O'Brien Kidney Center Cell Biology and Imaging Core
6. O'Brien Kidney Center Clinical and Translational Core
7. MRI Tools and References

Contacts