The Structural Biology Laboratory (SBL) provides access and assistance with the techniques of X-ray crystallography, small angle X-ray scattering and high resolution imaging as well as single particle reconstruction in cryo-electron microscopy. For X-ray this includes assistance with crystal screening and optimization, in-house X-ray diffraction screening and data collection, synchrotron data collection at the Advanced Photon Source (APS) for single crystals and small angle X-ray scattering (SAXS) measurements. For single particle cryo-EM, this includes assistance with protein sample preparation, grid preparation for negative staining and cryo-EM, grid optimization, collection, processing and analysis of data from in-house or remote microscopes, structure determination and validation. SBL is on-track to offer Micro-crystal Electron Diffraction (microED) service in 2022.
Diana Tomchick, Ph.D.
Director
214-645-6383
Diana.Tomchick@UTSouthwestern.edu
Zhe (James) Chen, Ph.D.
co-Director on Cryo-EM
214-648-1207
Yang Li, Ph.D.
Assitant Professor
214-645-5849
Helen Aronovich
Senior Research Associate
214-645-6382
Helen.Aronovich@UTSouthwestern.edu
Hours | Location |
Monday - Friday 9 AM - 5 PM 24/7 Availability for trained instrument usage |
ND Building, floors 8 & 10 6001 Forest Park Rd. Dallas, TX 75390-8816 |
Name | Role | Phone | Location | |
---|---|---|---|---|
Diana Tomchick, Ph.D. |
Director
|
214-645-6383
|
Diana.Tomchick@UTSouthwestern.edu
|
ND10.214A
|
Zhe (James) Chen, Ph.D. |
co-Director
|
214-648-1207
|
Zhe.Chen@UTSouthwestern.edu
|
ND10.214F
|
Service List |
► Consumables, EM (9) | |||
Name | Description | Price | |
---|---|---|---|
C-clip and ring (sold as a pair) |
for clipping cryo-EM grid to form an Autogrid
|
Internal
$23.50
each
UT System/In Network $23.50 each External $47.00 each UTSW Generic $58.75 each Commercial/Industry $70.50 each |
|
EM grid, Quantifoil/copper |
for cryo-EM data collection
|
Internal
$10.25
each
UT System/In Network $10.25 each External $20.50 each UTSW Generic $25.65 each Commercial/Industry $30.75 each |
|
EM grid, Quantifoil/gold |
for cryo-EM data collection |
Inquire | |
EM grid, copper |
for negative stain EM
|
Internal
$3.25
each
UT System/In Network $3.25 each External $6.50 each Commercial/Industry $9.75 each UTSW Generic $8.15 each |
|
EM grid, lacey carbon, 300 mesh, copper |
for cryo-EM data collection |
Inquire | |
EM grid, C-flat | Inquire | ||
Grid box, cryo, holds 4 Autogrids |
for storage of frozen, clipped grids in liquid nitrogen
|
Internal
$15.50
each
UT System/In Network $15.50 each External $31.00 each Commercial/Industry $46.50 each UTSW Generic $38.75 each |
|
Grid box, holds 4 unclipped grids |
for storage of frozen, unclipped grids in liquid nitrogen.
|
Internal
$10.25
each
UT System/In Network $10.25 each External $20.50 each Commercial/Industry $30.75 each UTSW Generic $25.65 each |
|
Mica (Per sheet) |
for carbon coated grid preparation
|
Internal
$5.00
each
UT System/In Network $5.00 each External $10.00 each Commercial/Industry $15.00 each UTSW Generic $12.50 each |
|
► Consumables, X-ray (12) | |||
Name | Description | Price | |
Robotic crystallization consumables |
Screen includes all consumables: tray, screen and sealing tapes.
|
Internal
$31.50
each
UT System/In Network $31.50 each External $63.00 each Commercial/Industry $94.50 each UTSW Generic $78.75 each |
|
96-well robotic crystallization screen |
Charge if user supplies their own robotic crystallization plate
|
Internal
$23.50
each
UT System/In Network $23.50 each External $47.00 each Commercial/Industry $70.50 each UTSW Generic $58.75 each |
|
Protein filters |
0.22 micron filter for proteins
|
Internal
$4.25
each
UT System/In Network $4.25 each External $8.50 each Commercial/Industry $12.75 each UTSW Generic $10.65 each |
|
24-well crystallization optimization tray (VDX) |
Includes all consumables
|
Internal
$36.50
each
UT System/In Network $36.50 each External $73.00 each Commercial/Industry $109.50 each UTSW Generic $91.25 each |
|
24-well crystallization sitting drop tray, Cryschem |
Includes all consumables
|
Internal
$20.00
each
UT System/In Network $20.00 each External $40.00 each Commercial/Industry $60.00 each UTSW Generic $50.00 each |
|
48-well crystallization optimization tray |
Includes all consumables
|
Internal
$43.00
each
UT System/In Network $43.00 each External $86.00 each Commercial/Industry $129.00 each UTSW Generic $107.50 each |
|
96-well sitting drop tray (Corning) |
Includes all consumables |
Internal
$40.00
each
UT System/In Network $40.00 each External $80.00 each Commercial/Industry $120.00 each UTSW Generic $100.00 each |
|
15-well crystallization optimization tray, Nextal |
Includes all consumables
|
Internal
$40.25
each
UT System/In Network $40.25 each External $80.50 each Commercial/Industry $120.75 each UTSW Generic $100.65 each |
|
24-well crystallization optimization tray, Nextal |
Includes all consumables
|
Internal
$41.00
each
UT System/In Network $41.00 each External $82.00 each Commercial/Industry $123.00 each UTSW Generic $102.50 each |
|
24-4 well crystallization sitting drop tray, Intelliplate |
Includes all consumables
|
Internal
$27.50
each
UT System/In Network $27.50 each External $55.00 each Commercial/Industry $82.50 each UTSW Generic $68.75 each |
|
12 mm glass coverslips, siliconized (Per pack) |
Pack of 240 coverslips
|
Internal
$70.25
each
UT System/In Network $70.25 each External $140.50 each Commercial/Industry $210.75 each UTSW Generic $175.65 each |
|
22 mm glass coverslips, siliconized (Per pack) |
Pack of 122 coverslips
|
Internal
$43.00
each
UT System/In Network $43.00 each External $86.00 each Commercial/Industry $129.00 each UTSW Generic $107.50 each |
|
► Data collection & structure determination, EM (15) | |||
Name | Description | Price | |
Grid screening, cryo-EM, Talos Arctica, assisted |
Price includes per hour charges for instrument usage as determined by the CEMF (Cryo-EM Facility). |
Internal
$96.50
each
UT System/In Network $96.50 each External $193.00 each Commercial/Industry $354.50 each UTSW Generic $298.75 each |
|
Grid screening, cryo-EM, Talos Arctica, unassisted |
Only available to internal users.
These are the per hour charges for microscope time, as determined by the CEMF (Cryo-EM Facility).
|
UT System/In Network
$35.00
each
External $70.00 each Commercial/Industry $170.00 each UTSW Generic $145.00 each |
|
Data collection, cryo-EM, Talos Arctica, assisted setup |
This is a flat fee; price does NOT include per hour instrument usage.
|
Internal
$220.00
each
UT System/In Network $220.00 each External $440.00 each Commercial/Industry $660.00 each UTSW Generic $550.00 each |
|
Grid screening, cryo-EM, Titan Krios, assisted |
Price includes per hour charges for instrument usage as determined by the CEMF (Cryo-EM Facility). |
Inquire | |
Grid screening, cryo-EM, Titan Krios, unassisted |
Only available to internal users.
These are the per hour charges for microscope time, as determined by the CEMF (Cryo-EM Facility). |
Internal
$40.00
each
UT System/In Network $40.00 each External $80.00 each Commercial/Industry $210.00 each UTSW Generic $175.00 each |
|
Data collection, cryo-EM, Titan Krios, assisted setup |
This is a flat fee; price does NOT include the per hour instrument usage. |
Internal
$275.00
each
UT System/In Network $275.00 each External $550.00 each Commercial/Industry $825.00 each UTSW Generic $687.50 each |
|
Initial data analysis and data processing, EM, assisted |
From movies to 2D classification.
Including motion correction (if needed) -> CTF estimate -> particle picking/selection -> 2D classification/analysis.
Please note: The rates listed below are cumulative and are based on a straightforward SPA workflow with a homogeneous dataset. Additional charges at an hourly rate of $60/hour would be added for more difficult projects. Users would be notified beforehand under such circumstances.
|
Internal
$255.00
each
UT System/In Network $255.00 each External $510.00 each Commercial/Industry $765.00 each UTSW Generic $637.50 each |
|
Additional data processing, EM |
From 2D to 3D refinement/postprocessing.
Including 2D class selection-> 3D initial model -> 3D classification/analysis -> 3D refinement -> postprocessing and analysis with FSC curves.
Please note: The rates listed below are cumulative and are based on a straightforward SPA workflow with a homogeneous dataset. Additional charges at an hourly rate per user classification would be added for more difficult projects. Users would be notified beforehand under such circumstances.
|
Internal
$410.00
each
UT System/In Network $410.00 each External $820.00 each Commercial/Industry $1,230.00 each UTSW Generic $1,025.00 each |
|
Advanced data processing |
Final CTF refinement and particle polishing (requires Relion motion correction step).
Please note: The rates listed are cumulative and are based on a straightforward SPA workflow with a homogeneous dataset. Additional charges at an hourly rate per user classification would be added for more difficult projects. Users would be notified beforehand under such circumstances.
|
Internal
$410.00
each
UT System/In Network $410.00 each External $820.00 each Commercial/Industry $1,230.00 each UTSW Generic $1,025.00 each |
|
Manuscript preparation, EM |
Includes generation of figures and tables, description of methods, manuscript text, deposition of coordinates in database (PDB and/or EMDB). |
Internal
$155.00
each
UT System/In Network $155.00 each External $310.00 each Commercial/Industry $465.00 each UTSW Generic $387.50 each |
|
EM specimen preparation for cryo-EM, assisted |
For cryo-EM screening and data collection.
Includes per hour charge for Vitrobot usage.
|
Internal
$87.00
each
UT System/In Network $87.00 each External $174.00 each Commercial/Industry $261.00 each UTSW Generic $217.50 each |
|
EM Specimen preparation for cryo-EM, unassisted |
For cryo-EM screening and data collection.
This is the per hour charge for Vitrobot usage.
|
Internal
$25.50
each
UT System/In Network $25.50 each External $51.00 each Commercial/Industry $76.50 each UTSW Generic $63.75 each |
|
EM Specimen preparation for negative staining, assisted |
For screening samples prior to screening by cryo-EM data and/or data collection.
|
Internal
$71.75
each
UT System/In Network $71.75 each External $143.50 each Commercial/Industry $261.00 each UTSW Generic $179.40 each |
|
EM Specimen preparation for negative staining, unassisted |
For screening samples prior to screening by cryo-EM data and/or data collection.
|
Internal
$10.25
each
UT System/In Network $10.25 each External $20.50 each Commercial/Industry $30.75 each UTSW Generic $25.65 each |
|
Data collection or grid screening, negative stain EM, 120kV TEM, assisted |
Price includes a $50/hour instrument usage for internal users.
Instrument usage costs are higher for other users.
|
Internal
$111.50
each
UT System/In Network $111.50 each External $223.00 each Commercial/Industry $334.50 each UTSW Generic $278.75 each |
|
► Data collection and structure determination, X-ray (10) | |||
Name | Description | Price | |
Crystal shipping to synchrotron via FedEx |
Sample shipping both to and from synchrotron via FedEx.
Prices are per sample.
Service not available for commercial customers.
|
Internal
$3.50
each
UT System/In Network $3.50 each |
|
Synchrotron Data Collection and Processing, X-ray, assisted |
Assisted collection, processing and scaling of synchrotron single crystal X-ray data, per dataset.
Service not available to commercial customers.
|
Internal
$155.00
each
UT System/In Network $155.00 each |
|
SAXS sample shipping to synchrotron via FedEx |
Determined after trip is finished; costs to be shared by all users during specific data collection time.
Service not available to commercial customers.
|
Internal
$1.00
each
UT System/In Network $1.00 each |
|
Synchrotron SAXS Data Collection |
Collection in one buffer @ 3 concentrations.
Service not available to commercial customers.
|
Internal
$155.00
each
UT System/In Network $155.00 each |
|
MicroMax-003 w/ACTOR robot for crystal screening |
Robotic crystal screening of frozen samples.
Prices are per hour basis.
Assisted usage only.
|
Internal
$25.00
each
UT System/In Network $25.00 each External $50.00 each Commercial/Industry $75.00 each UTSW Generic $62.50 each |
|
MicroMax-003 w/XtalCheck |
for in situ screening and data collection of 96-condition crystallization screen plates.
Prices are per hour basis.
Assisted usage only.
Room temperature only.
|
Internal
$25.00
each
UT System/In Network $25.00 each External $50.00 each Commercial/Industry $75.00 each UTSW Generic $62.50 each |
|
In-house data collection and processing, X-ray, assisted |
Assisted data collection, processing and scaling of in-house single-crystal data, per dataset
|
Internal
$51.00
each
UT System/In Network $51.00 each External $102.00 each Commercial/Industry $153.00 each UTSW Generic $127.50 each |
|
Structure determination and refinement |
Project Specific.
|
Internal
$510.00
each
UT System/In Network $510.00 each External $1,020.00 each Commercial/Industry $1,530.00 each UTSW Generic $1,275.00 each |
|
Manuscript preparation, X-ray |
Internal
$250.00
each
UT System/In Network $250.00 each External $500.00 each Commercial/Industry $750.00 each UTSW Generic $625.00 each |
||
Small Molecule structure determination |
Includes data collection, structure determination, refinement and delivery of all data required for publication.
|
Inquire | |
► Instrument Use, X-ray (2) | |||
Name | Description | Price | |
Crystallization robot (Per tray) |
Trained or self use of crystallization robot; users will be charged for per plate usage, see charges under "Consumables, X-ray". |
Internal
$31.50
plate
UT System/In Network $31.50 plate External $63.00 plate Commercial/Industry $94.50 plate UTSW Generic $78.75 each |
|
MicroMax-003 data collection |
Prices are per hour basis.
|
Internal
$25.00
/hr
UT System/In Network $25.00 /hr External $50.00 /hr Commercial/Industry $75.00 /hr UTSW Generic $62.50 each |