Welcome!
ALERT As of December 31st, 2019, the TLS 1.0 protocol will no longer be supported by Agilent, including older browsers (e.g. IE 10, Safari 6, Firefox 26 and older) and users will not have access to the iLab sites. Please click here for more information.
The Structural Biology Laboratory (SBL) provides access and assistance with the techniques of X-ray crystallography and single particle cryo-electron microscopy reconstruction, and assistance with structure determination, refinement and interpretation. For X-ray this includes assistance with crystal screening and optimization, in-house X-ray diffraction screening and data collection, synchrotron data collection at the Advanced Photon Source (APS) for single crystals and Small Angle X-ray Scattering (SAXS) measurements. For single particle cryo-EM, this includes assistance with protein sample preparation, grid preparation for negative staining and cryo-EM, grid optimization, collection, processing and analysis of data from in-house microscopes, structure determination and validation.
Diana Tomchick, Ph.D.
Director
214-645-6383
Diana.Tomchick@UTSouthwestern.edu
Zhe (James) Chen, Ph.D.
214-648-1207
Yang Li, Ph.D.
214-645-5849
Helen Aronovich
Research Technician
214-645-6382
Helen.Aronovich@UTSouthwestern.edu
| Hours | Location |
|
Monday - Friday 9 AM - 5 PM 24/7 Availability for trained instrument usage |
ND Building, floors 8 & 10 6001 Forest Park Rd. Dallas, TX 75390-8816 |
| Name | Role | Phone | Location | |
|---|---|---|---|---|
| Diana Tomchick, Ph.D. |
Director
|
214-645-6383
|
Diana.Tomchick@UTSouthwestern.edu
|
ND10.214A
|
| Service List |
| ► Consumables, EM (7) | |||
| Name | Description | Price | |
|---|---|---|---|
| C-clip and ring (sold as a pair) |
for clipping cryo-EM grid to form an Autogrid
|
Internal
$22.00
each
UT System/In Network $44.00 each External $55.00 each Commercial/Industry $66.00 each |
|
| EM grid, AutoGrid |
clipped EM grid, for Cryo-EM data collection.
Specifically, this is a Quantifoil/copper grid plus C-clip & ring.
|
Internal
$32.00
each
UT System/In Network $64.00 each External $80.00 each Commercial/Industry $96.00 each |
|
| EM grid, Quantifoil/copper |
for cryo-EM data collection
|
Internal
$10.00
each
UT System/In Network $20.00 each External $25.00 each Commercial/Industry $30.00 each |
|
| EM grid, copper |
for negative stain EM
|
Internal
$3.00
each
UT System/In Network $6.00 each External $7.50 each Commercial/Industry $9.00 each |
|
| Grid box, cryo, holds 4 Autogrids |
for storage of frozen, clipped grids in liquid nitrogen
|
Internal
$15.00
each
UT System/In Network $30.00 each External $37.50 each Commercial/Industry $45.00 each |
|
| Grid box, holds 4 unclipped grids |
for storage of frozen, unclipped grids in liquid nitrogen.
|
Internal
$10.00
each
UT System/In Network $20.00 each External $25.00 each Commercial/Industry $30.00 each |
|
| Mica (Per sheet) |
for carbon coated grid preparation
|
Internal
$5.00
each
UT System/In Network $10.00 each External $12.50 each Commercial/Industry $15.00 each |
|
| ► Consumables, X-ray (12) | |||
| Name | Description | Price | |
| Robotic crystallization consumables |
Screen includes all consumables: tray, screen and sealing tapes.
|
Internal
$30.00
each
UT System/In Network $60.00 each External $75.00 each Commercial/Industry $90.00 each |
|
| 96-well robotic crystallization screen |
Charge if user supplies their own robotic crystallization plate
|
Internal
$23.00
each
UT System/In Network $46.00 each External $57.50 each Commercial/Industry $69.00 each |
|
| Protein filters |
0.22 micron filter for proteins
|
Internal
$4.00
each
UT System/In Network $8.00 each External $10.00 each Commercial/Industry $12.00 each |
|
| 24-well crystallization optimization tray (VDX) |
Includes all consumables
|
Internal
$36.00
each
UT System/In Network $72.00 each External $90.00 each Commercial/Industry $108.00 each |
|
| 24-well crystallization sitting drop tray, Cryschem |
Includes all consumables
|
Internal
$26.00
each
UT System/In Network $52.00 each External $65.00 each Commercial/Industry $78.00 each |
|
| 48-well crystallization optimization tray |
Includes all consumables
|
Internal
$40.00
each
UT System/In Network $80.00 each External $100.00 each Commercial/Industry $120.00 each |
|
| 96-well sitting drop tray (Corning) |
Includes all consumables |
Internal
$30.00
each
UT System/In Network $60.00 each External $75.00 each Commercial/Industry $90.00 each |
|
| 15-well crystallization optimization tray, Nextal |
Includes all consumables
|
Internal
$30.00
each
UT System/In Network $60.00 each External $75.00 each Commercial/Industry $90.00 each |
|
| 24-well crystallization optimization tray, Nextal |
Includes all consumables
|
Internal
$48.00
each
UT System/In Network $96.00 each External $120.00 each Commercial/Industry $144.00 each |
|
| 24-4 well crystallization sitting drop tray, Intelliplate |
Includes all consumables
|
Internal
$20.00
each
UT System/In Network $40.00 each External $60.00 each Commercial/Industry $80.00 each |
|
| 12 mm glass coverslips, siliconized (Per pack) |
Pack of 240 coverslips
|
Internal
$65.00
each
UT System/In Network $130.00 each External $162.50 each Commercial/Industry $195.00 each |
|
| 22 mm glass coverslips, siliconized (Per pack) |
Pack of 122 coverslips
|
Internal
$42.00
each
UT System/In Network $84.00 each External $105.00 each Commercial/Industry $126.00 each |
|
| ► Data collection & structure determination, EM (10) | |||
| Name | Description | Price | |
| Data collection or grid screening, cryo-EM, Titan Krios, assisted |
Price includes $30/hour instrument usage for internal users.
Instrument usage is higher for all other users. |
Inquire | |
| Data collection or grid screening, cryo-EM, Titan Krios, unassisted |
Only available to internal users. |
Inquire | |
| Data collection or grid screening, cryo-EM, Talos Arctica, assisted |
Price includes $25/hour instrument usage for internal users.
Instrument usage for all other users is higher.
|
Inquire | |
| EM specimen preparation for cryo-EM, assisted |
for cryo-EM screening and data collection.
Includes per hour charge for Vitrobot usage.
|
Internal
$85.00
each
UT System/In Network $170.00 each External $212.50 each Commercial/Industry $255.00 each |
|
| EM Specimen preparation for cryo-EM, unassisted |
for cryo-EM screening and data collection.
This is the per hour charge for Vitrobot usage.
|
Inquire | |
| Data analysis and data processing, EM, assisted |
Assisted data analysis and processing, up to and including 2D classification, per dataset
|
Internal
$250.00
each
UT System/In Network $500.00 each External $625.00 each Commercial/Industry $750.00 each |
|
| EM Specimen preparation for negative staining, assisted |
for screening samples of cryo-EM data collection
|
Internal
$30.00
each
UT System/In Network $60.00 each External $75.00 each Commercial/Industry $90.00 each |
|
| EM Specimen preparation for negative staining, unassisted |
for screening samples of cryo-EM data collection
|
Inquire | |
| Data collection or grid screening, negative stain EM, 120kV TEM, assisted |
Price includes a $50/hour instrument usage for internal users.
Instrument usage costs are higher for other users.
|
Internal
$110.00
each
UT System/In Network $220.00 each External $275.00 each Commercial/Industry $330.00 each |
|
| Protein Sample optimization for EM, assisted |
Preparation of protein sample for EM, assisted
|
Internal
$4.00
each
UT System/In Network $8.00 each External $10.00 each Commercial/Industry $12.00 each |
|
| ► Data collection and structure determination, X-ray (7) | |||
| Name | Description | Price | |
| Crystal shipping to synchrotron via FedEx |
Sample shipping both to and from synchrotron via FedEx.
Prices are per sample.
Service not available for commercial customers.
|
Internal
$3.00
each
UT System/In Network $6.00 each External $7.50 each |
|
| MicroMax-003 w/ACTOR robot for crystal screening |
Robotic crystal screening of frozen samples.
Prices are per hour basis.
Assisted usage only.
|
Internal
$20.00
each
UT System/In Network $40.00 each External $50.00 each Commercial/Industry $60.00 each |
|
| MicroMax-003 w/XtalCheck |
for in situ screening and data collection of 96-condition crystallization screen plates.
Prices are per hour basis.
Assisted usage only.
Room temperature only.
|
Internal
$20.00
each
UT System/In Network $40.00 each External $50.00 each Commercial/Industry $60.00 each |
|
| SAXS sample shipping to synchrotron via FedEx |
Determined after trip is finished; costs to be shared by all users during specific data collection time.
Service not available to commercial customers.
|
Internal
$1.00
each
UT System/In Network $2.00 each External $2.50 each |
|
| Structure determination, refinement and publication |
Project Specific.
|
Internal
$500.00
each
UT System/In Network $1,000.00 each External $1,250.00 each Commercial/Industry $1,500.00 each |
|
| Synchrotron Data Collection and Processing, X-ray, assisted |
Assisted collection, processing and scaling of synchrotron single crystal X-ray data, per dataset.
|
Internal
$150.00
each
UT System/In Network $300.00 each External $375.00 each Commercial/Industry $450.00 each |
|
| Synchrotron SAXS Data Collection |
Collection in one buffer @ 3 concentrations.
Service not available to commercial customers.
|
Internal
$150.00
each
UT System/In Network $300.00 each External $375.00 each |
|
| ► Instrument Use, X-ray (2) | |||
| Name | Description | Price | |
| Crystallization robot (Per tray) |
Trained or self use of crystallization robot; users will be charged for per plate usage, see charges under "Consumables, X-ray". |
Internal
$30.00
plate
UT System/In Network $60.00 plate External $75.00 plate Commercial/Industry $90.00 plate |
|
| MicroMax-003 data collection |
Prices are per hour basis.
|
Internal
$20.00
/hr
UT System/In Network $40.00 /hr External $50.00 /hr Commercial/Industry $60.00 /hr |
|
