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The Structural Biology Laboratory (SBL) provides access and assistance with the techniques of X-ray crystallography and single particle cryo-electron microscopy reconstruction, and assistance with structure determination, refinement and interpretation. For X-ray this includes assistance with crystal screening and optimization, in-house X-ray diffraction screening and data collection, synchrotron data collection at the Advanced Photon Source (APS) for single crystals and Small Angle X-ray Scattering (SAXS) measurements. For single particle cryo-EM, this includes assistance with protein sample preparation, grid preparation for negative staining and cryo-EM, grid optimization, collection, processing and analysis of data from in-house microscopes, structure determination and validation.
Diana Tomchick, Ph.D.
Director
214-645-6383
Diana.Tomchick@UTSouthwestern.edu
Zhe (James) Chen, Ph.D.
214-648-1207
Yang Li, Ph.D.
214-645-5849
Helen Aronovich
Research Technician
214-645-6382
Helen.Aronovich@UTSouthwestern.edu
| Hours | Location |
|
Monday - Friday 9 AM - 5 PM 24/7 Availability for trained instrument usage |
ND Building, floors 8 & 10 6001 Forest Park Rd. Dallas, TX 75390-8816 |
| Name | Role | Phone | Location | |
|---|---|---|---|---|
| Diana Tomchick, Ph.D. |
Director
|
214-645-6383
|
Diana.Tomchick@UTSouthwestern.edu
|
ND10.214A
|
| Service List |
| ► Consumables, EM (7) | |||
| Name | Description | Price | |
|---|---|---|---|
| C-clip and ring (sold as a pair) |
for clipping cryo-EM grid to form an Autogrid
|
Internal
$22.00
each
UT System/In Network $44.00 each External $55.00 each Commercial/Industry $66.00 each |
|
| EM grid, AutoGrid |
clipped EM grid, for Cryo-EM data collection.
Specifically, this is a Quantifoil/copper grid plus C-clip & ring.
|
Internal
$32.00
each
UT System/In Network $64.00 each External $80.00 each Commercial/Industry $96.00 each |
|
| EM grid, Quantifoil/copper |
for cryo-EM data collection
|
Internal
$10.00
each
UT System/In Network $20.00 each External $25.00 each Commercial/Industry $30.00 each |
|
| EM grid, copper |
for negative stain EM
|
Internal
$3.00
each
UT System/In Network $6.00 each External $7.50 each Commercial/Industry $9.00 each |
|
| Grid box, cryo, holds 4 Autogrids |
for storage of frozen, clipped grids in liquid nitrogen
|
Internal
$15.00
each
UT System/In Network $30.00 each External $37.50 each Commercial/Industry $45.00 each |
|
| Grid box, holds 4 unclipped grids |
for storage of frozen, unclipped grids in liquid nitrogen.
|
Internal
$10.00
each
UT System/In Network $20.00 each External $25.00 each Commercial/Industry $30.00 each |
|
| Mica (Per sheet) |
for carbon coated grid preparation
|
Internal
$5.00
each
UT System/In Network $10.00 each External $12.50 each Commercial/Industry $15.00 each |
|
| ► Consumables, X-ray (12) | |||
| Name | Description | Price | |
| Robotic crystallization consumables |
Screen includes all consumables: tray, screen and sealing tapes.
|
Internal
$30.00
each
UT System/In Network $60.00 each External $75.00 each Commercial/Industry $90.00 each |
|
| 96-well robotic crystallization screen |
Charge if user supplies their own robotic crystallization plate
|
Internal
$23.00
each
UT System/In Network $46.00 each External $57.50 each Commercial/Industry $69.00 each |
|
| Protein filters |
0.22 micron filter for proteins
|
Internal
$4.00
each
UT System/In Network $8.00 each External $10.00 each Commercial/Industry $12.00 each |
|
| 24-well crystallization optimization tray (VDX) |
Includes all consumables
|
Internal
$36.00
each
UT System/In Network $72.00 each External $90.00 each Commercial/Industry $108.00 each |
|
| 24-well crystallization sitting drop tray, Cryschem |
Includes all consumables
|
Internal
$26.00
each
UT System/In Network $52.00 each External $65.00 each Commercial/Industry $78.00 each |
|
| 48-well crystallization optimization tray |
Includes all consumables
|
Internal
$40.00
each
UT System/In Network $80.00 each External $100.00 each Commercial/Industry $120.00 each |
|
| 96-well sitting drop tray (Corning) |
Includes all consumables |
Internal
$30.00
each
UT System/In Network $60.00 each External $75.00 each Commercial/Industry $90.00 each |
|
| 15-well crystallization optimization tray, Nextal |
Includes all consumables
|
Internal
$30.00
each
UT System/In Network $60.00 each External $75.00 each Commercial/Industry $90.00 each |
|
| 24-well crystallization optimization tray, Nextal |
Includes all consumables
|
Internal
$48.00
each
UT System/In Network $96.00 each External $120.00 each Commercial/Industry $144.00 each |
|
| 24-4 well crystallization sitting drop tray, Intelliplate |
Includes all consumables
|
Internal
$20.00
each
UT System/In Network $40.00 each External $60.00 each Commercial/Industry $80.00 each |
|
| 12 mm glass coverslips, siliconized (Per pack) |
Pack of 240 coverslips
|
Internal
$65.00
each
UT System/In Network $130.00 each External $162.50 each Commercial/Industry $195.00 each |
|
| 22 mm glass coverslips, siliconized (Per pack) |
Pack of 122 coverslips
|
Internal
$42.00
each
UT System/In Network $84.00 each External $105.00 each Commercial/Industry $126.00 each |
|
| ► Data collection & structure determination, EM (10) | |||
| Name | Description | Price | |
| Data collection or grid screening, cryo-EM, Titan Krios, assisted |
Price includes $30/hour instrument usage for internal users.
Instrument usage is higher for all other users. |
Inquire | |
| Data collection or grid screening, cryo-EM, Titan Krios, unassisted |
Only available to internal users. |
Inquire | |
| Data collection or grid screening, cryo-EM, Talos Arctica, assisted |
Price includes $25/hour instrument usage for internal users.
Instrument usage for all other users is higher.
|
Inquire | |
| EM specimen preparation for cryo-EM, assisted |
for cryo-EM screening and data collection.
Includes per hour charge for Vitrobot usage.
|
Internal
$85.00
each
UT System/In Network $170.00 each External $212.50 each Commercial/Industry $255.00 each |
|
| EM Specimen preparation for cryo-EM, unassisted |
for cryo-EM screening and data collection.
This is the per hour charge for Vitrobot usage.
|
Inquire | |
| Data analysis and data processing, EM, assisted |
Assisted data analysis and processing, up to and including 2D classification, per dataset
|
Internal
$250.00
each
UT System/In Network $500.00 each External $625.00 each Commercial/Industry $750.00 each |
|
| EM Specimen preparation for negative staining, assisted |
for screening samples of cryo-EM data collection
|
Internal
$30.00
each
UT System/In Network $60.00 each External $75.00 each Commercial/Industry $90.00 each |
|
| EM Specimen preparation for negative staining, unassisted |
for screening samples of cryo-EM data collection
|
Inquire | |
| Data collection or grid screening, negative stain EM, 120kV TEM, assisted |
Price includes a $50/hour instrument usage for internal users.
Instrument usage costs are higher for other users.
|
Internal
$110.00
each
UT System/In Network $220.00 each External $275.00 each Commercial/Industry $330.00 each |
|
| Protein Sample optimization for EM, assisted |
Preparation of protein sample for EM, assisted
|
Internal
$4.00
each
UT System/In Network $8.00 each External $10.00 each Commercial/Industry $12.00 each |
|
| ► Data collection and structure determination, X-ray (7) | |||
| Name | Description | Price | |
| Crystal shipping to synchrotron via FedEx |
Sample shipping both to and from synchrotron via FedEx.
Prices are per sample.
Service not available for commercial customers.
|
Internal
$3.00
each
UT System/In Network $6.00 each External $7.50 each |
|
| MicroMax-003 w/ACTOR robot for crystal screening |
Robotic crystal screening of frozen samples.
Prices are per hour basis.
Assisted usage only.
|
Internal
$20.00
each
UT System/In Network $40.00 each External $50.00 each Commercial/Industry $60.00 each |
|
| MicroMax-003 w/XtalCheck |
for in situ screening and data collection of 96-condition crystallization screen plates.
Prices are per hour basis.
Assisted usage only.
Room temperature only.
|
Internal
$20.00
each
UT System/In Network $40.00 each External $50.00 each Commercial/Industry $60.00 each |
|
| SAXS sample shipping to synchrotron via FedEx |
Determined after trip is finished; costs to be shared by all users during specific data collection time.
Service not available to commercial customers.
|
Internal
$1.00
each
UT System/In Network $2.00 each External $2.50 each |
|
| Structure determination, refinement and publication |
Project Specific.
|
Internal
$500.00
each
UT System/In Network $1,000.00 each External $1,250.00 each Commercial/Industry $1,500.00 each |
|
| Synchrotron Data Collection and Processing, X-ray, assisted |
Assisted collection, processing and scaling of synchrotron single crystal X-ray data, per dataset.
|
Internal
$150.00
each
UT System/In Network $300.00 each External $375.00 each Commercial/Industry $450.00 each |
|
| Synchrotron SAXS Data Collection |
Collection in one buffer @ 3 concentrations.
Service not available to commercial customers.
|
Internal
$150.00
each
UT System/In Network $300.00 each External $375.00 each |
|
| ► Instrument Use, X-ray (2) | |||
| Name | Description | Price | |
| Crystallization robot (Per tray) |
Trained or self use of crystallization robot; users will be charged for per plate usage, see charges under "Consumables, X-ray". |
Internal
$30.00
plate
UT System/In Network $60.00 plate External $75.00 plate Commercial/Industry $90.00 plate |
|
| MicroMax-003 data collection |
Prices are per hour basis.
|
Internal
$20.00
/hr
UT System/In Network $40.00 /hr External $50.00 /hr Commercial/Industry $60.00 /hr |
|
