Welcome!
ALERT Alert: Users running reports may encounter an error, when filtering the date field by Billing Date, after the iLab US server migration. Agilent Engineers are working to resolve as soon as possible. In the meantime, users can try reducing the date range of these reports (eg. 1-2 month intervals), to load them.
Status of this issue can be tracked on the iLab Issue Tracker, when you click on Help at the top right of your iLab account > under North American iLab Sites (except Canada, DFCI and Stanford) > (OPEN) Reporting by "Billing Date" Date Field Filter Not Loading.
The Structural Biology Laboratory (SBL) provides access and assistance with the techniques of X-ray crystallography and single particle cryo-electron microscopy reconstruction, and assistance with structure determination, refinement and interpretation. For X-ray this includes assistance with crystal screening and optimization, in-house X-ray diffraction screening and data collection, synchrotron data collection at the Advanced Photon Source (APS) for single crystals and Small Angle X-ray Scattering (SAXS) measurements. For single particle cryo-EM, this includes assistance with protein sample preparation, grid preparation for negative staining and cryo-EM, grid optimization, collection, processing and analysis of data from in-house microscopes, structure determination and validation.
Diana Tomchick, Ph.D.
Director
214-645-6383
Diana.Tomchick@UTSouthwestern.edu
Zhe (James) Chen, Ph.D.
214-648-1207
Yang Li, Ph.D.
214-645-5849
Helen Aronovich
Research Technician
214-645-6382
Helen.Aronovich@UTSouthwestern.edu
| Hours | Location |
|
Monday - Friday 9 AM - 5 PM 24/7 Availability for trained instrument usage |
ND Building, floors 8 & 10 6001 Forest Park Rd. Dallas, TX 75390-8816 |
| Name | Role | Phone | Location | |
|---|---|---|---|---|
| Diana Tomchick, Ph.D. |
Director
|
214-645-6383
|
Diana.Tomchick@UTSouthwestern.edu
|
ND10.214A
|
| Service List |
| ► Consumables, EM (9) | |||
| Name | Description | Price | |
|---|---|---|---|
| C-clip and ring (sold as a pair) |
for clipping cryo-EM grid to form an Autogrid
|
Internal
$23.50
each
UT System/In Network $23.50 each External $47.00 each UTSW Generic $58.75 each Commercial/Industry $70.50 each |
|
| EM grid, Quantifoil/copper |
for cryo-EM data collection
|
Internal
$10.25
each
UT System/In Network $10.25 each External $20.50 each UTSW Generic $25.65 each Commercial/Industry $30.75 each |
|
| EM grid, Quantifoil/gold |
for cryo-EM data collection |
Inquire | |
| EM grid, copper |
for negative stain EM
|
Internal
$3.25
each
UT System/In Network $3.25 each External $6.50 each Commercial/Industry $9.75 each UTSW Generic $8.15 each |
|
| EM grid, lacey carbon, 300 mesh, copper |
for cryo-EM data collection |
Inquire | |
| EM grid, C-flat | Inquire | ||
| Grid box, cryo, holds 4 Autogrids |
for storage of frozen, clipped grids in liquid nitrogen
|
Internal
$15.50
each
UT System/In Network $15.50 each External $31.00 each Commercial/Industry $46.50 each UTSW Generic $38.75 each |
|
| Grid box, holds 4 unclipped grids |
for storage of frozen, unclipped grids in liquid nitrogen.
|
Internal
$10.25
each
UT System/In Network $10.25 each External $20.50 each Commercial/Industry $30.75 each UTSW Generic $25.65 each |
|
| Mica (Per sheet) |
for carbon coated grid preparation
|
Internal
$5.00
each
UT System/In Network $5.00 each External $10.00 each Commercial/Industry $15.00 each UTSW Generic $12.50 each |
|
| ► Consumables, X-ray (12) | |||
| Name | Description | Price | |
| Robotic crystallization consumables |
Screen includes all consumables: tray, screen and sealing tapes.
|
Internal
$31.50
each
UT System/In Network $31.50 each External $63.00 each Commercial/Industry $94.50 each UTSW Generic $78.75 each |
|
| 96-well robotic crystallization screen |
Charge if user supplies their own robotic crystallization plate
|
Internal
$23.50
each
UT System/In Network $23.50 each External $47.00 each Commercial/Industry $70.50 each UTSW Generic $58.75 each |
|
| Protein filters |
0.22 micron filter for proteins
|
Internal
$4.25
each
UT System/In Network $4.25 each External $8.50 each Commercial/Industry $12.75 each UTSW Generic $10.65 each |
|
| 24-well crystallization optimization tray (VDX) |
Includes all consumables
|
Internal
$36.50
each
UT System/In Network $36.50 each External $73.00 each Commercial/Industry $109.50 each UTSW Generic $91.25 each |
|
| 24-well crystallization sitting drop tray, Cryschem |
Includes all consumables
|
Internal
$20.00
each
UT System/In Network $20.00 each External $40.00 each Commercial/Industry $60.00 each UTSW Generic $50.00 each |
|
| 48-well crystallization optimization tray |
Includes all consumables
|
Internal
$43.00
each
UT System/In Network $43.00 each External $867.00 each Commercial/Industry $129.00 each UTSW Generic $107.50 each |
|
| 96-well sitting drop tray (Corning) |
Includes all consumables |
Internal
$40.00
each
UT System/In Network $40.00 each External $80.00 each Commercial/Industry $120.00 each UTSW Generic $100.00 each |
|
| 15-well crystallization optimization tray, Nextal |
Includes all consumables
|
Internal
$40.25
each
UT System/In Network $40.25 each External $80.50 each Commercial/Industry $120.75 each UTSW Generic $100.65 each |
|
| 24-well crystallization optimization tray, Nextal |
Includes all consumables
|
Internal
$41.00
each
UT System/In Network $41.00 each External $82.00 each Commercial/Industry $123.00 each UTSW Generic $102.50 each |
|
| 24-4 well crystallization sitting drop tray, Intelliplate |
Includes all consumables
|
Internal
$27.50
each
UT System/In Network $27.50 each External $55.00 each Commercial/Industry $82.50 each UTSW Generic $68.75 each |
|
| 12 mm glass coverslips, siliconized (Per pack) |
Pack of 240 coverslips
|
Internal
$70.25
each
UT System/In Network $70.25 each External $140.50 each Commercial/Industry $210.75 each UTSW Generic $175.65 each |
|
| 22 mm glass coverslips, siliconized (Per pack) |
Pack of 122 coverslips
|
Internal
$43.00
each
UT System/In Network $43.00 each External $86.00 each Commercial/Industry $129.00 each UTSW Generic $107.50 each |
|
| ► Data collection & structure determination, EM (16) | |||
| Name | Description | Price | |
| Grid screening, cryo-EM, Talos Arctica, assisted |
Price includes per hour charges for instrument usage as determined by the CEMF (Cryo-EM Facility). |
Internal
$91.50
each
UT System/In Network $91.50 each External $183.00 each Commercial/Industry $354.50 each UTSW Generic $298.75 each |
|
| Grid screening, cryo-EM, Talos Arctica, unassisted |
Only available to internal users.
These are the per hour charges for microscope time, as determined by the CEMF (Cryo-EM Facility).
|
UT System/In Network
$30.00
each
External $60.00 each Commercial/Industry $170.00 each UTSW Generic $145.00 each |
|
| Data collection, cryo-EM, Talos Arctica, assisted setup |
This is a flat fee; price does NOT include per hour instrument usage.
|
Internal
$205.00
each
UT System/In Network $205.00 each External $410.00 each Commercial/Industry $615.00 each UTSW Generic $512.50 each |
|
| Grid screening, cryo-EM, Titan Krios, assisted |
Price includes per hour charges for instrument usage as determined by the CEMF (Cryo-EM Facility). |
Inquire | |
| Grid screening, cryo-EM, Titan Krios, unassisted |
Only available to internal users.
These are the per hour charges for microscope time, as determined by the CEMF (Cryo-EM Facility). |
Internal
$35.00
each
UT System/In Network $35.00 each External $70.00 each Commercial/Industry $210.00 each UTSW Generic $175.00 each |
|
| Data collection, cryo-EM, Titan Krios, assisted setup |
This is a flat fee; price does NOT include the per hour instrument usage. |
Internal
$255.00
each
UT System/In Network $255.00 each External $510.00 each Commercial/Industry $765.00 each UTSW Generic $637.50 each |
|
| Initial data analysis and data processing, EM, assisted |
From movies to 2D classification.
Including motion correction (if needed) -> CTF estimate -> particle picking/selection -> 2D classification/analysis.
Please note: The rates listed below are cumulative and are based on a straightforward SPA workflow with a homogeneous dataset. Additional charges at an hourly rate of $60/hour would be added for more difficult projects. Users would be notified beforehand under such circumstances.
|
Internal
$255.00
each
UT System/In Network $255.00 each External $510.00 each Commercial/Industry $765.00 each UTSW Generic $637.50 each |
|
| Additional data processing, EM |
From 2D to 3D refinement/postprocessing.
Including 2D class selection-> 3D initial model -> 3D classification/analysis -> 3D refinement -> postprocessing and analysis with FSC curves.
Please note: The rates listed below are cumulative and are based on a straightforward SPA workflow with a homogeneous dataset. Additional charges at an hourly rate per user classification would be added for more difficult projects. Users would be notified beforehand under such circumstances.
|
Internal
$410.00
each
UT System/In Network $410.00 each External $820.00 each Commercial/Industry $1,230.00 each UTSW Generic $1,025.00 each |
|
| Advanced data processing |
Final CTF refinement and particle polishing (requires Relion motion correction step).
Please note: The rates listed are cumulative and are based on a straightforward SPA workflow with a homogeneous dataset. Additional charges at an hourly rate per user classification would be added for more difficult projects. Users would be notified beforehand under such circumstances.
|
Internal
$410.00
each
UT System/In Network $410.00 each External $820.00 each Commercial/Industry $1,230.00 each UTSW Generic $1,025.00 each |
|
| Manuscript preparation, EM |
Includes generation of figures and tables, description of methods, manuscript text, deposition of coordinates in database (PDB and/or EMDB). |
Internal
$155.00
each
UT System/In Network $155.00 each External $310.00 each Commercial/Industry $465.00 each UTSW Generic $387.50 each |
|
| EM specimen preparation for cryo-EM, assisted |
For cryo-EM screening and data collection.
Includes per hour charge for Vitrobot usage.
|
Internal
$87.00
each
UT System/In Network $87.00 each External $174.00 each Commercial/Industry $261.00 each UTSW Generic $217.50 each |
|
| EM Specimen preparation for cryo-EM, unassisted |
For cryo-EM screening and data collection.
This is the per hour charge for Vitrobot usage.
|
Internal
$25.50
each
UT System/In Network $25.50 each External $51.00 each Commercial/Industry $76.50 each UTSW Generic $63.75 each |
|
| EM Specimen preparation for negative staining, assisted |
For screening samples prior to screening by cryo-EM data and/or data collection.
|
Internal
$71.75
each
UT System/In Network $71.75 each External $143.50 each Commercial/Industry $261.00 each UTSW Generic $179.40 each |
|
| EM Specimen preparation for negative staining, unassisted |
For screening samples prior to screening by cryo-EM data and/or data collection.
|
Internal
$10.25
each
UT System/In Network $10.25 each External $20.50 each Commercial/Industry $30.75 each UTSW Generic $25.65 each |
|
| Data collection or grid screening, negative stain EM, 120kV TEM, assisted |
Price includes a $50/hour instrument usage for internal users.
Instrument usage costs are higher for other users.
|
Internal
$111.50
each
UT System/In Network $111.50 each External $223.00 each Commercial/Industry $334.50 each UTSW Generic $278.75 each |
|
| Protein Sample optimization for EM, assisted |
Preparation of protein sample for EM, assisted
|
Internal
$4.25
each
UT System/In Network $4.25 each External $8.50 each Commercial/Industry $12.75 each UTSW Generic $10.65 each |
|
| ► Data collection and structure determination, X-ray (10) | |||
| Name | Description | Price | |
| Crystal shipping to synchrotron via FedEx |
Sample shipping both to and from synchrotron via FedEx.
Prices are per sample.
Service not available for commercial customers.
|
Internal
$3.50
each
UT System/In Network $3.50 each |
|
| Synchrotron Data Collection and Processing, X-ray, assisted |
Assisted collection, processing and scaling of synchrotron single crystal X-ray data, per dataset.
Service not available to commercial customers.
|
Internal
$155.00
each
UT System/In Network $155.00 each |
|
| SAXS sample shipping to synchrotron via FedEx |
Determined after trip is finished; costs to be shared by all users during specific data collection time.
Service not available to commercial customers.
|
Internal
$1.00
each
UT System/In Network $1.00 each |
|
| Synchrotron SAXS Data Collection |
Collection in one buffer @ 3 concentrations.
Service not available to commercial customers.
|
Internal
$155.00
each
UT System/In Network $155.00 each |
|
| MicroMax-003 w/ACTOR robot for crystal screening |
Robotic crystal screening of frozen samples.
Prices are per hour basis.
Assisted usage only.
|
Internal
$25.00
each
UT System/In Network $25.00 each External $50.00 each Commercial/Industry $75.00 each UTSW Generic $62.50 each |
|
| MicroMax-003 w/XtalCheck |
for in situ screening and data collection of 96-condition crystallization screen plates.
Prices are per hour basis.
Assisted usage only.
Room temperature only.
|
Internal
$25.00
each
UT System/In Network $25.00 each External $50.00 each Commercial/Industry $75.00 each UTSW Generic $62.50 each |
|
| In-house data collection and processing, X-ray, assisted |
Assisted data collection, processing and scaling of in-house single-crystal data, per dataset
|
Internal
$51.00
each
UT System/In Network $51.00 each External $102.00 each Commercial/Industry $153.00 each UTSW Generic $127.50 each |
|
| Structure determination and refinement |
Project Specific.
|
Internal
$510.00
each
UT System/In Network $510.00 each External $1,020.00 each Commercial/Industry $1,530.00 each UTSW Generic $1,275.00 each |
|
| Manuscript preparation, X-ray |
Internal
$155.00
each
UT System/In Network $155.00 each External $310.00 each Commercial/Industry $465.00 each UTSW Generic $387.50 each |
||
| Small Molecule structure determination |
Includes data collection, structure determination, refinement and delivery of all data required for publication.
|
Inquire | |
| ► Instrument Use, X-ray (2) | |||
| Name | Description | Price | |
| Crystallization robot (Per tray) |
Trained or self use of crystallization robot; users will be charged for per plate usage, see charges under "Consumables, X-ray". |
Internal
$31.50
plate
UT System/In Network $31.50 plate External $63.00 plate Commercial/Industry $94.50 plate UTSW Generic $78.75 each |
|
| MicroMax-003 data collection |
Prices are per hour basis.
|
Internal
$25.00
/hr
UT System/In Network $25.00 /hr External $50.00 /hr Commercial/Industry $75.00 /hr UTSW Generic $62.50 each |
|
