Welcome!
The Structural Biology Laboratory (SBL) provides access and assistance with the techniques of X-ray crystallography, small angle X-ray scattering and high resolution imaging as well as single particle reconstruction in cryo-electron microscopy. For X-ray this includes assistance with crystal screening and optimization, in-house X-ray diffraction screening and data collection, synchrotron data collection at the Advanced Photon Source (APS) for single crystals and small angle X-ray scattering (SAXS) measurements. For single particle cryo-EM, this includes assistance with protein sample preparation, grid preparation for negative staining and cryo-EM, grid optimization, collection, processing and analysis of data from in-house or remote microscopes, structure determination and validation. SBL is on-track to offer Micro-crystal Electron Diffraction (microED) service in 2022.
Diana Tomchick, Ph.D.
Professor & Director
214-645-6383
Diana.Tomchick@UTSouthwestern.edu
Zhe (James) Chen, Ph.D.
Professor & co-Director on Cryo-EM
214-648-1207
Yang Li, Ph.D.
Assitant Professor
214-645-5849
Yan Han, Ph.D.
Assitant Professor
214-645-9888
Helen Aronovich
Senior Research Associate
214-645-6382
Helen.Aronovich@UTSouthwestern.edu
Amber Flores
Research Assistant II
214-645-6382
Amber.Flores@UTSouthwestern.edu
| Hours | Location |
|
Monday - Friday 9 AM - 5 PM 24/7 Availability for trained instrument usage |
ND Building, floors 8 & 10 6001 Forest Park Rd. Dallas, TX 75390-8816 |
| Name | Role | Phone | Location | |
|---|---|---|---|---|
| Diana Tomchick, Ph.D. |
Director
|
214-645-6383
|
Diana.Tomchick@UTSouthwestern.edu
|
ND10.214A
|
| Zhe (James) Chen, Ph.D. |
co-Director
|
214-648-1207
|
Zhe.Chen@UTSouthwestern.edu
|
ND10.214F
|
| Service List |
| ► Consumables, EM (11) | |||
| Name | Description | Price | |
|---|---|---|---|
| EM grid, Quantifoil/copper with Graphene Oxide |
for cryo-EM data collection |
Inquire | |
| EM grid, Quantifoil/gold with Graphene Oxide |
for cryo-EM data collection |
Inquire | |
| C-clip and ring (sold as a pair) |
for clipping cryo-EM grid to form an Autogrid
|
Internal
$24.00
each
UT System/In Network $24.00 each External $48.00 each UTSW Generic $60.00 each Commercial/Industry $72.00 each |
|
| EM grid, Quantifoil/copper |
for cryo-EM data collection
|
Internal
$10.50
each
UT System/In Network $10.50 each External $21.00 each UTSW Generic $26.25 each Commercial/Industry $31.50 each |
|
| EM grid, Quantifoil/gold |
for cryo-EM data collection |
Inquire | |
| EM grid, copper |
for negative stain EM
|
Internal
$3.50
each
UT System/In Network $3.50 each External $7.00 each UTSW Generic $8.75 each Commercial/Industry $10.50 each |
|
| EM grid, lacey carbon, 300 mesh, copper |
for cryo-EM data collection |
Inquire | |
| EM grid, C-flat | Inquire | ||
| Grid box, cryo, holds 4 Autogrids |
for storage of frozen, clipped grids in liquid nitrogen
|
Internal
$15.80
each
UT System/In Network $15.80 each External $31.60 each Commercial/Industry $47.40 each UTSW Generic $39.50 each |
|
| Grid box, holds 4 unclipped grids |
for storage of frozen, unclipped grids in liquid nitrogen.
|
Internal
$10.50
each
UT System/In Network $10.50 each External $21.00 each Commercial/Industry $31.50 each UTSW Generic $26.25 each |
|
| Mica (Per sheet) |
for carbon coated grid preparation
|
Internal
$5.10
each
UT System/In Network $5.10 each External $10.20 each Commercial/Industry $15.30 each UTSW Generic $12.75 each |
|
| ► Consumables, X-ray (12) | |||
| Name | Description | Price | |
| Robotic crystallization consumables |
Screen includes all consumables: tray, screen and sealing tapes.
|
Internal
$32.15
each
UT System/In Network $31.15 each External $64.30 each Commercial/Industry $96.45 each UTSW Generic $80.40 each |
|
| 96-well robotic crystallization screen |
Charge if user supplies their own robotic crystallization plate
|
Internal
$24.00
each
UT System/In Network $24.00 each External $48.00 each Commercial/Industry $72.00 each UTSW Generic $60.00 each |
|
| Protein filters |
0.22 micron filter for proteins
|
Internal
$4.35
each
UT System/In Network $4.35 each External $8.70 each Commercial/Industry $13.05 each UTSW Generic $10.85 each |
|
| 24-well crystallization optimization tray (VDX) |
Includes all consumables
|
Internal
$37.25
each
UT System/In Network $37.25 each External $74.50 each Commercial/Industry $111.75 each UTSW Generic $93.15 each |
|
| 24-well crystallization sitting drop tray, Cryschem |
Includes all consumables
|
Internal
$20.40
each
UT System/In Network $20.40 each External $40.80 each Commercial/Industry $61.20 each UTSW Generic $51.00 each |
|
| 48-well crystallization optimization tray |
Includes all consumables
|
Internal
$43.85
each
UT System/In Network $43.85 each External $87.70 each Commercial/Industry $131.55 each UTSW Generic $109.65 each |
|
| 96-well sitting drop tray (Corning) |
Includes all consumables |
Internal
$40.80
each
UT System/In Network $40.80 each External $81.60 each Commercial/Industry $122.40 each UTSW Generic $102.00 each |
|
| 15-well crystallization optimization tray, Nextal |
Includes all consumables
|
Internal
$41.10
each
UT System/In Network $41.10 each External $82.20 each Commercial/Industry $123.30 each UTSW Generic $102.75 each |
|
| 24-well crystallization optimization tray, Nextal |
Includes all consumables
|
Internal
$41.80
each
UT System/In Network $41.80 each External $83.60 each Commercial/Industry $125.40 each UTSW Generic $104.50 each |
|
| 24-4 well crystallization sitting drop tray, Intelliplate |
Includes all consumables
|
Internal
$28.05
each
UT System/In Network $28.05 each External $56.10 each Commercial/Industry $84.15 each UTSW Generic $70.15 each |
|
| 12 mm glass coverslips, siliconized (Per pack) |
Pack of 240 coverslips
|
Internal
$71.65
each
UT System/In Network $71.65 each External $143.30 each Commercial/Industry $214.95 each UTSW Generic $179.15 each |
|
| 22 mm glass coverslips, siliconized (Per pack) |
Pack of 122 coverslips
|
Internal
$43.85
each
UT System/In Network $43.85 each External $87.70 each Commercial/Industry $131.55 each UTSW Generic $109.65 each |
|
| ► Data collection & structure determination, EM (17) | |||
| Name | Description | Price | |
| Grid screening, cryo-EM, 200 kV microscope, assisted |
Price includes per hour charges for instrument usage as determined by the CEMF (Cryo-EM Facility). |
Internal
$100.75
each
UT System/In Network $100.75 each External $201.50 each Commercial/Industry $361.25 each UTSW Generic $305.00 each |
|
| cryo-EM, 200 kV microscope, unassisted |
Only available to internal users.
These are the per hour charges for microscope time, as determined by the CEMF (Cryo-EM Facility).
|
UT System/In Network
$38.00
each
External $76.00 each Commercial/Industry $173.00 each UTSW Generic $148.00 each |
|
| Data collection, cryo-EM, 200 kV microscope, assisted setup |
This is a flat fee; price does NOT include per hour instrument usage.
|
Internal
$224.50
each
UT System/In Network $224.50 each External $449.00 each Commercial/Industry $673.50 each UTSW Generic $561.25 each |
|
| Grid screening, cryo-EM, Titan Krios, assisted |
Price includes per hour charges for instrument usage as determined by the CEMF (Cryo-EM Facility). |
Inquire | |
| cryo-EM, Titan Krios, unassisted |
Only available to internal users.
These are the per hour charges for microscope time, as determined by the CEMF (Cryo-EM Facility). |
Internal
$46.00
each
UT System/In Network $46.00 each External $92.00 each Commercial/Industry $216.00 each UTSW Generic $181.00 each |
|
| Data collection, cryo-EM, Titan Krios, assisted setup |
This is a flat fee; price does NOT include the per hour instrument usage. |
Internal
$280.50
each
UT System/In Network $280.50 each External $561.00 each Commercial/Industry $841.50 each UTSW Generic $701.25 each |
|
| Initial data analysis and data processing, EM, assisted |
From movies to 2D classification.
Including motion correction (if needed) -> CTF estimate -> particle picking/selection -> 2D classification/analysis.
Please note: The rates listed below are cumulative and are based on a straightforward SPA workflow with a homogeneous dataset. Additional charges at an hourly rate of $60/hour would be added for more difficult projects. Users would be notified beforehand under such circumstances.
|
Internal
$418.00
each
UT System/In Network $418.00 each External $836.00 each Commercial/Industry $1,254.00 each UTSW Generic $1,045.00 each |
|
| Additional data processing, EM |
From 2D to 3D refinement/postprocessing.
Including 2D class selection-> 3D initial model -> 3D classification/analysis -> 3D refinement -> postprocessing and analysis with FSC curves.
Please note: The rates listed below are cumulative and are based on a straightforward SPA workflow with a homogeneous dataset. Additional charges at an hourly rate per user classification would be added for more difficult projects. Users would be notified beforehand under such circumstances.
|
Internal
$418.00
each
UT System/In Network $418.00 each External $836.00 each Commercial/Industry $1,254.00 each UTSW Generic $1,045.00 each |
|
| Advanced data processing |
Final CTF refinement and particle polishing (requires Relion motion correction step).
Please note: The rates listed are cumulative and are based on a straightforward SPA workflow with a homogeneous dataset. Additional charges at an hourly rate per user classification would be added for more difficult projects. Users would be notified beforehand under such circumstances.
|
Internal
$418.00
each
UT System/In Network $418.00 each External $836.00 each Commercial/Industry $1,254.00 each UTSW Generic $1,045.00 each |
|
| Manuscript preparation, EM |
Includes generation of figures and tables, description of methods, manuscript text, deposition of coordinates in database (PDB and/or EMDB). |
Internal
$158.00
each
UT System/In Network $158.00 each External $316.00 each Commercial/Industry $474.00 each UTSW Generic $395.00 each |
|
| cryo-EM grid preparation, assisted |
Includes per hour charge for Vitrobot, plasma cleaner and autoGrid station usage.
|
Internal
$88.75
each
UT System/In Network $88.75 each External $177.50 each Commercial/Industry $266.25 each UTSW Generic $221.85 each |
|
| Vitrobot usage, unassisted |
Internal
$26.00
each
UT System/In Network $26.00 each External $52.00 each Commercial/Industry $78.00 each UTSW Generic $65.00 each |
||
| EM Specimen preparation for negative staining, assisted |
For screening samples prior to screening by cryo-EM data and/or data collection.
|
Internal
$73.20
each
UT System/In Network $73.20 each External $146.40 each Commercial/Industry $219.60 each UTSW Generic $183.00 each |
|
| EM Specimen preparation for negative staining, unassisted |
For screening samples prior to screening by cryo-EM data and/or data collection.
|
Internal
$10.50
each
UT System/In Network $10.50 each External $21.00 each Commercial/Industry $31.50 each UTSW Generic $26.25 each |
|
| Data collection or grid screening, negative stain EM, 120kV TEM, assisted |
Price includes a $50/hour instrument usage for internal users.
Instrument usage costs are higher for other users.
|
Internal
$113.75
each
UT System/In Network $113.75 each External $227.50 each Commercial/Industry $341.25 each UTSW Generic $284.40 each |
|
| Dewar shipment via FedEx to offsite location |
Covers dewar preparation services and FedEx charges. |
Internal
$250.00
each
UT System/In Network $250.00 each External $500.00 each Commercial/Industry $750.00 each UTSW Generic $625.00 each |
|
| Dewar preparation for FedEx shipping |
Covers dewar preparation services only. |
Internal
$70.00
each
UT System/In Network $70.00 each External $140.00 each Commercial/Industry $210.00 each UTSW Generic $175.00 each |
|
| ► Data collection and structure determination, X-ray (10) | |||
| Name | Description | Price | |
| Crystal shipping to synchrotron via FedEx |
Sample shipping both to and from synchrotron via FedEx.
Prices are per sample.
Service not available for commercial customers.
|
Internal
$3.60
each
UT System/In Network $3.60 each External $7.20 each |
|
| Synchrotron Data Collection and Processing, X-ray, assisted |
Assisted collection, processing and scaling of synchrotron single crystal X-ray data, per dataset.
Service not available to commercial customers.
|
Internal
$158.00
each
UT System/In Network $158.00 each External $316.00 each |
|
| SAXS sample shipping to synchrotron via FedEx |
Determined after trip is finished; costs to be shared by all users during specific data collection time.
Service not available to commercial customers.
|
Internal
$1.00
each
UT System/In Network $1.00 each External $2.00 each |
|
| Synchrotron SAXS Data Collection |
Collection in one buffer @ 3 concentrations.
Service not available to commercial customers.
|
Internal
$158.00
each
UT System/In Network $158.00 each External $316.00 each |
|
| MicroMax-003 w/ACTOR robot for crystal screening |
Robotic crystal screening of frozen samples.
Prices are per hour basis.
Assisted usage only.
|
Internal
$25.50
each
UT System/In Network $25.50 each External $51.00 each Commercial/Industry $76.50 each UTSW Generic $63.75 each |
|
| MicroMax-003 w/XtalCheck |
for in situ screening and data collection of 96-condition crystallization screen plates.
Prices are per hour basis.
Assisted usage only.
Room temperature only.
|
Internal
$25.50
each
UT System/In Network $25.50 each External $51.00 each Commercial/Industry $76.50 each UTSW Generic $63.75 each |
|
| In-house data collection and processing, X-ray, assisted |
Assisted data collection, processing and scaling of in-house single-crystal data, per dataset
|
Internal
$52.00
each
UT System/In Network $52.00 each External $104.00 each Commercial/Industry $156.00 each UTSW Generic $130.00 each |
|
| Structure determination and refinement |
Project Specific.
|
Internal
$520.00
each
UT System/In Network $520.00 each External $1,040.00 each Commercial/Industry $1,560.00 each UTSW Generic $1,300.00 each |
|
| Manuscript preparation, X-ray |
Internal
$255.00
each
UT System/In Network $255.00 each External $510.00 each Commercial/Industry $765.00 each UTSW Generic $637.50 each |
||
| Small Molecule structure determination |
Includes data collection, structure determination, refinement and delivery of all data required for publication.
|
Internal
$255.00
each
UT System/In Network $255.00 each External $510.00 each Commercial/Industry $765.00 each UTSW Generic $637.50 each |
|
| ► Instrument Use, X-ray (2) | |||
| Name | Description | Price | |
| Crystallization robot (Per tray) |
Trained or self use of crystallization robot; users will be charged for per plate usage, see charges under "Consumables, X-ray". |
Internal
$32.15
plate
UT System/In Network $32.15 plate External $64.30 plate Commercial/Industry $96.45 plate UTSW Generic $80.40 each |
|
| MicroMax-003 data collection |
Prices are per hour basis.
|
Internal
$25.50
/hr
UT System/In Network $25.50 /hr External $51.00 /hr Commercial/Industry $76.50 /hr UTSW Generic $63.75 each |
|
