UTSW Metabolic Phenotyping Core

NEWS

Starting on 3-18-2020 and during UTSW shut-down period.

Scintillation counter will be taken off-line

We will only be accepting samples that can be stored for a prolonged   period of time at -20 °C or -80°C.

Analysis requests for unstable metabolites are not accepted.

List of unstable metabolites (freezing samples is not recommended):

Serum: Plasma: NEFA, ALT, LDH and AMYL

Urine: AMYL, BUN, UPRO and URIC

* Please contact ruth.gordillo@utsouthwestern.edu or richard.wynn@utsouthwestern.edu to schedule your sample drop-off time.

Thank you for your cooperation and stay safe.

Effective Immediately (01-06-2019)

The Metabolic Phenotyping Core offering now complete portfolio of analytical services:


*VITROS analysis requests from Monday-Friday and rush orders.
*FPLC Serum/Plasma Lipoprotein Particles Fractionation.
*Urine Collection Cages for mice.
*Milliplex, Cytokines.
*SeaHorse Flux Analyzer

Effective Immediately (11-21-2019)
 
To use the scintillation counter please make reservations using the iLab portal (24 hours in advance).

*Scintillation counter ONLY available during business hours (M-F 9:00 am-5:30 pm. Not available during holidays).

*Please make sure you sign-in the log spreadsheet located next to the instrument.

LOCATION L4-102

 Updated Core Hours of Operation: Mon-Fri (9:00 AM - 5:30 PM) 

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VITROS no sample drop-off on Fridays after 3:00 pm.

For analysis of unstable metabolites (NEFA, Total ketones, 3HB, ALKP, ALT, LDH UPRO) no sample drop-off on Fridays after 12:00 noon. Late sample submission fee will be applied (2x $price). Effective immediately (06-15-2018)

Rush order analysis additional fees will apply (2x$price) (01-01-2018)

NEW!! Please be aware of cancellation fees for CLAMP SERVICES.  Effective Immediately (11-01-2017)

Billing cycles occur at monthly intervals ususally in the first week of the following month. Please make sure your subledger will not be expired at the time of billing.

Thank you for your cooperation.

Overview of Services

The Metabolic Phenotyping Core (MMPC) provides state-of-the-art analytical and phenotypical measures to the scientific community both inside and outside UT Southwestern Medical Center.

Our goal is to expand the scope of techniques available to investigators, standardize key methodologies, and expedite the completion of research on diseases related to metabolic disorders (diabetes and obesity), cancer, aging neurological disorders, etc.

• Chemistry analysis using VITROS MicroSlide™ technology, ELISAs, and magnetic bead mapping multiplex technology
• Tissue extraction of cholesterol and triglyceride level
• Metabolic chambers for indirect gas calorimetry, activity, and food/water consumption
• Urine collection chambers
• Body composition analysis (MRI )
• Glucose-insulin clamp studies
• Seahorse XF-24(3) Extracellular Flux Analyzer for cellular metabolism studies
• Primary hepatocyte and β-cell isolation from mice or rats
• Sphingolipid profiling using LC-MS/MS technology
• Free Amiono Acids profiling using LC-MS/MS technology. For Metabolic preclinical and clinical studies (Not for diagnosis purposes).
• Exercise treadmills for mice and rats
• RNA Scope and Base Scope Hybridization

Leadership

Philipp Scherer, PhD.

Professor, PI

Philipp.Scherer@UTSouthwestern.edu

Joel Elmquist, D.V.M., PhD.

Professor, PI

joel.elmquist@UTSouthwestern.edu

Jay Horton, M.D.

Professor, PI

jay.horton@UTSouthwestern.edu

Ruth Gordillo, Ph.D.

Director

214-648-2566

Ruth.Gordillo@UTSouthwestern.edu

Syann Lee, Ph.D.

Co-Director

214-648-3782

Syann.Lee@UTSouthwestern.edu

Richard (Max) Wynn, Ph.D.

Support Faculty

214-645-4405

Richard.Wynn@UTSouthwestern.edu

Staff 

Shannon Hacker

Research Technician II

214-645-4405

Shannon.Hacker@utsouthwestern.edu

Allison Walker

Research Technician II

214-645-4405

Allison.Walker@utsouthwestern.edu

Location and hours of operation

Links and Resources

1. http://touchstonelabs.org/metabolic-phenotyping-core/
2. https://www.utsouthwestern.edu/research/core-facilities/
3. http://www.utsouthwestern.edu/education/medical-school/departments/internal-medicine/divisions/touchstone-diabetes/index.html

Contacts