ALERT

In Observance of U.S. Holiday, Memorial Day, Agilent Cross Lab/ iLab Operations Software Support Help Desk will be closed during U.S. hours on Monday May 25th, 2020. We will resume regular U.S. support hours on Tuesday May 26th, 2020. EU and APAC Support will remain open during this time. For urgent matters, please add "Urgent" to the ticket/ email subject or press "1", when prompted, to escalate a call on the iLab Support phone and we will prioritize those requests first.

UTSW Metabolic Phenotyping Core

NEWS

05-12-2020

Dear Metabolic Phenotyping Core Users,

*Starting on Wednesday May 13th 2020, the Metabolic Phenotyping core will be accepting sample for VITROS analysis of stable Metabolites. Sample submission schedule Monday-Friday 9:00-12:00

*Starting on Monday May 18th 2020, the Metabolic Phenotyping core will be accepting sample for VITROS analysis of all kinds of analysis. Sample submission schedule Monday-Friday 9:00-12:00

For samples to be stored at 4 °C please use the mini-fridge allocated next to the entrance door (NMR scan door)

For samples to be stored at -20 °C please use the usual VITROS freezer.

Please maintain social distancing at all times. The core will be operating at 25% capacity and the personnel will be working on rotating shifts. Consultation services and questions will be addressed exclusively via email.

As a reminder:

VITROS analysis unstable metabolites (storing samples in the freezer not recommended): ALT, LDH, AMYL, UPRO and Uric Acid in Urine Samples.

05-05-2020

Dear Metabolic Phenotyping Core Users,

Starting on May 11th 2020, the Metabolic Phenotyping core will be accepting sample submissions again for the assays listed below:

  • Tissue Extractions
  • NEFA
  • ELISA
  • Milliplex Cytokine
  • Mass Spectrometry assays
  • SeaHorse Experiments
  • Bomb Calorimetry
  • Plasma Lipoprotein Fractionation
  • RNA Scope

Sample submission schedule: Monday-Friday 9:00am-12:00 noon.

Please maintain social distancing at all times. The core will be operating at 25% capacity and the personnel will be working on rotating shifts. Consultation services and questions will be addressed exclusively via email.

VITROS Chemistry analyses will resume as soon as the instrument performance is validated. Updates will be posted on the Ilab portal landing pages.

Instrument usage scheduling.

  • Mouse NMR scanning (body composition). Scheduling by user available 24 hours in advance. Please make sure you are on time
  • Plate reader. Contact core personnel.
  • Scintillation counter. Only available reduced number of hours. Please contact core personnel.
  • SeaHorse Experiments. Contact core personnel for scheduling and planning.

Core Contacts for analytical lab

Ruth Gordillo, Ph.D.        Ruth.gordillo@utsouthwestern.edu

Richard (Max) Wynn, Ph.D.          Richard.wynn@utsouthwestern.edu

Shannon Hacker               Shannon.hacker@utsouthwestern.edu

Allison Walker                   Allison.walker@utsouthwestern.edu

Core Contacts for metabolic cages and treadmills 

Syann Lee, Ph.D.               Syann.lee@utsouthwestern.edu

Core Contacts for surgeries, RNAscope and BaseScope hybridization

Laurent Gautron, Ph.D.                  Laurent.Gautron@utsouthwestern.edu 

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Effective Immediately (01-06-2019)

The Metabolic Phenotyping Core offering now complete portfolio of analytical services:


*VITROS analysis requests from Monday-Friday and rush orders.
*FPLC Serum/Plasma Lipoprotein Particles Fractionation.
*Urine Collection Cages for mice.
*Milliplex, Cytokines.
*SeaHorse Flux Analyzer


Effective Immediately (11-21-2019)


To use the scintillation counter please make reservations using the iLab portal (24 hours in advance).

*Scintillation counter ONLY available during business hours (M-F 9:00 am-5:30 pm. Not available during holidays).


*Please make sure you sign-in the log spreadsheet located next to the instrument.

 

 

LOCATION L4-102

 Updated Core Hours of Operation: Mon-Fri (9:00 AM - 5:30 PM) 

 

 

 

 

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VITROS no sample drop-off on Fridays after 3:00 pm.

For analysis of unstable metabolites (NEFA, Total ketones, 3HB, ALKP, ALT, LDH UPRO) no sample drop-off on Fridays after 12:00 noon. Late sample submission fee will be applied (2x $price). Effective immediately (06-15-2018)

Rush order analysis additional fees will apply (2x$price) (01-01-2018)

 

 

NEW!! Please be aware of cancellation fees for CLAMP SERVICES.  Effective Immediately (11-01-2017)

Billing cycles occur at monthly intervals ususally in the first week of the following month. Please make sure your subledger will not be expired at the time of billing.

Thank you for your cooperation.

 

Overview of Services

The Metabolic Phenotyping Core (MMPC) provides state-of-the-art analytical and phenotypical measures to the scientific community both inside and outside UT Southwestern Medical Center.

 

Our goal is to expand the scope of techniques available to investigators, standardize key methodologies, and expedite the completion of research on diseases related to metabolic disorders (diabetes and obesity), cancer, aging neurological disorders, etc.

  • Chemistry analysis using VITROS MicroSlide™ technology, ELISAs, and magnetic bead mapping multiplex technology
  • Tissue extraction of cholesterol and triglyceride level
  • Metabolic chambers for indirect gas calorimetry, activity, and food/water consumption
  • Urine collection chambers
  • Body composition analysis (MRI )
  • Glucose-insulin clamp studies
  • Seahorse XF-24(3) Extracellular Flux Analyzer for cellular metabolism studies
  • Primary hepatocyte and β-cell isolation from mice or rats
  • Sphingolipid profiling using LC-MS/MS technology
  • Free Amiono Acids profiling using LC-MS/MS technology. For Metabolic preclinical and clinical studies (Not for diagnosis purposes).
  • Exercise treadmills for mice and rats
  • RNA Scope and Base Scope Hybridization

Leadership

Philipp Scherer, PhD.

Professor, PI

Philipp.Scherer@UTSouthwestern.edu

 

Joel Elmquist, D.V.M., PhD.

Professor, PI

joel.elmquist@UTSouthwestern.edu

 

Jay Horton, M.D.

Professor, PI

jay.horton@UTSouthwestern.edu

  

Ruth Gordillo, Ph.D.

Director

214-648-2566

Ruth.Gordillo@UTSouthwestern.edu

 

Syann Lee, Ph.D.

Co-Director

214-648-3782

Syann.Lee@UTSouthwestern.edu

 

Richard (Max) Wynn, Ph.D.

Support Faculty

214-645-4405

Richard.Wynn@UTSouthwestern.edu

 

 

Staff 

 

 

Shannon Hacker

Research Technician II

214-645-4405

Shannon.Hacker@utsouthwestern.edu

 

Allison Walker

Research Technician II

214-645-4405

Allison.Walker@utsouthwestern.edu

  

Location and hours of operation

Hours Location

Staffed:

Monday - Friday     

9:00 AM - 5:30 PM

5323 Harry Hines Blvd.

Room L4.102

Dallas, Texas 75390

Links and Resources

  1. http://touchstonelabs.org/metabolic-phenotyping-core/
  2. https://www.utsouthwestern.edu/research/core-facilities/
  3. http://www.utsouthwestern.edu/education/medical-school/departments/internal-medicine/divisions/touchstone-diabetes/index.html

Contacts

Name Role Phone Email Location
Ruth Gordillo, Ph.D.
Director
 
214-648-2566
 
Ruth.Gordillo@UTSouthwestern.edu
 
K5-252
 
Syann Lee, Ph.D.
Co-Director
 
214-648-3782
 
Syann.Lee@UTsouthwestern.edu
 
Y6-220A
 
Christine Kusminski, Ph.D.
Support Faculty
 
214-648-8717
 
Christine.Kusminski@UTSouthwestern.edu
 
K5-244
 
Laurent Gautron, Ph.D.
Support Faculty
 
214-648-0272
 
Laurent.Gautron@UTSouthwestern.edu
 
Y6-220C
 
Shannon Hacker
Technical Personnel
 
214-645-4405
 
Shannon.Hacker@UTSouthwestern.edu
 
L4-102
 
Allison Walker
Technical Personnel
 
214-645-4405
 
Allison.Walker@utsouthwestern.edu
 
L4-102
 
Richard (Max) Wynn
Support Faculty
 
214-645-4405
 
Richard.Wynn@UTSouthwestern.edu
 
L4-102
 

Price List


Search available services: View: by category alphabetically
VITROS Services (4)
Sphingolipids Profiling Services (1)
Instrument Usage (1)
Fre Amino Acids (5)
NEFA (4)