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UTSW Metabolomics Core

 

Overview of Services



The Metabolomics Core Facility within the Biochemistry Department was established in 2017 by Dr. Hamid Baniasadi. The Core is an analytic facility that uses mass spectrometry techniques for measuring small molecules (metabolites) in biological samples such as cell line, tissue and body fluids. The Metabolomics Core at Biochemistry offers the following services:

  1.   Relative quantitation of metabolites (up to 150 polar metabolites) (LC-MS/MS analysis) (Triple Quad 6500, Sciex): Our service includes targeting of 289 metabolites using positive and negative ionization mode with multiple reaction monitoring (MRM). We are able to detect up to 150 polar metabolites in a biological sample.


  2.   Absolute quantitation of a single metabolite (LC-MS/MS analysis) (Triple Quad 6500, Sciex): In an absolute quantitation of metabolites, the core focuses on targeting a specific metabolite or group of metabolites for quantitation using heavy internal standards.


  3.   Small molecule analysis for exact mass determination (LC-MS analysis) (TripleTOF® 6600 System, Sciex): We offer exact mass measurement with less than 5-10 ppm mass accuracy, data processing and generation of the report.


  4.   Method Development: We develop new assay per user requests.


  5.   Data processing, Data Analysis, and Consulting



In 2019, we want to extend our service as follows:

  1.    Untargeted Metabolomics using TripleTOF® 6600 System, Sciex: Untargeted mass spectrometry (MS) based metabolomics analysis focuses on global detection and relative quantification of metabolites in a biological sample. This approach can be used for biomarker discovery and also identification of metabolic pathways which might contribute to treatments and diseases.
  2.   Metabolic Flux Analysis: The approach is carried out by feeding cells an isotopically labeled metabolite such as 13C-glucose and measuring the flux or rate of a particular metabolic process. Metabolic Flux Analysis can be used to investigate specific metabolites in metabolic pathways.

Consultation with Dr. Baniasadi regarding sample preparation and experimental design is recommended before sample preparation and design of experiment.

 



Metabolomics Fee is as follows:

  1.   Required $21,144 per year fixed buy in fee per department to contribute to salary expenses.
  2.   Individual faculty in departments that have paid the buy in fee will then pay for services using the below fee schedule:

   -Relative quantitation of metabolites (up to 150 polar metabolites) (LC-MS/MS analysis) (Triple Quad 6500, Sciex): Our service includes targeting of 289 metabolites using positive and negative ionization mode with multiple reaction monitoring (MRM). We are able to detect up to 150 polar metabolites in a biological sample.
        Cost: Fixed fee $25 per injection plus 20 cents per metabolite for data processing and data analysis. Experienced users that demonstrate proficiency can analyze their own data if agreed on by the PI.
   -Absolute quantitation of a single metabolites (LC-MS/MS analysis) (Triple Quad 6500, Sciex)
        Cost: Methods development. One-time fee $1,000. After that $25 per injection plus 50 cents per transition for data processing and data analysis. Heavy internal standard will be provided by users. Experienced users that   demonstrate proficiency can analyze their own data if agreed on by the PI.
   -Small molecule analysis for exact mass determination (LC-MS analysis) (TripleTOF® 6600 System, Sciex) : We offer exact mass measurement with less than 5-10 ppm mass accuracy , data processing, and generation of the report. This service is used to verify the product of a synthesis.
        Cost: $25 per sample.

 

 

 

Customers are invited to view the metabolomics service offerings for an idea of costs and details of service but exact pricing will be determined after consultation with Dr. Baniasadi.

 

Leadership

Hamid Baniasadi, Ph.D
Core Director
Hamid.Baniasadi@Utsouthwestern.edu
L4.256B

 

Location and hours of operation

Hours Location
Monday - Friday 
8:00 am - 5:00 pm 

 

5323 Harry Hines Boulevard

Room L4.308

Dallas, TX 75390-9038

Links and Resources

  1. Metabolomics Core Website

Contacts

Name Role Phone Email Location
Hamid Baniasadi, Ph.D
Core Director
 
214-648-6927
 
Hamid.Baniasadi@Utsouthwestern.edu
 
L4.256B
 
Thomas Perez

 
(214)648-9965
 
Thomas.perez2@utsouthwestern.edu
 
Y4.310
 

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