In 2018-19, UT Southwestern embarked on an ambitious gene therapy program, the future of curative medicine, by establishing Viral Vector Core lab and GMP AAV Viral Vector production facility to serve its own clinical studies and the community at large. Through building a professional team of unique expertise and collaborative efforts with multiple stakeholders, our goal is to establish a highly efficient full-service viral vector production facility that can respond timely to provide AAV-based research reagents and services to investigators at UT Southwestern, as well as to facilitate translational research applying gene therapy approaches to treat genetic disease. The objective is to deliver the highest quality AAV vectors to our academic, government, foundation, and biotech industry clients.
Mission
Our primary mission is to serve the research community at UT Southwestern by providing AAV vectors and associated services for gene therapy translational studies. Through dedicated efforts of highly skilled professional investigators, collaborators and foundations, we will bring the future of gene-based medicine into the present.
Vision
To be a leading gene therapy program, locally, nationally and globally.
Team Values
Production Facility
The Translational Gene Therapy Core (TGTC) - previously known as Viral Vector Core - is located on the 4th floor of the Green Research Building (Building Y) on UT Southwestern’s south campus. The space includes separate suites for Process Development, Quality Control (Y4.206) and GMP AAV Viral Vector production facility (Y4.230).
Our Production Platform
Our production process utilizes triple-transfection of suspension HEK293 cells, using serum-free media. Our GMP production suite can utilize a 500 L bioreactor for a single production run. Downstream purification of AAV relies on filtration, concentration, and chromatography steps without the use of density gradients. We maintain consistency of our manufacturing process across all production scales (0.5 L to 500 L), to ensure comparability of the product throughout the translational pipeline.
Steven Gray, Ph.D. | Director, Translational Gene Therapy Core (TGTC)
steven.gray@utsouthwestern.edu
214-648-0670
Samer Dahshi, M.S. | TGTC - Operations Manager
samer.dahshi@utsouthwestern.edu
214-648-7164
Hours | Location |
Monday - Friday 8am - 5pm |
Y04.214 |
Name | Role | Phone | Location | |
---|---|---|---|---|
Steven Gray, Ph.D. |
Director,Translational Gene Therapy Core (TGTC)
|
214-648-0670
|
steven.gray@utsouthwestern.edu
|
NA02.508AA
|
Samer Dahshi, M.S. |
Operations Manager - TGTC
|
214-648-7164
|
samer.dahshi@utsouthwestern.edu
|
Y04.214
|
Service list |
► Production (11) | |||
Name | Description | Price | |
---|---|---|---|
0.5L-scale research AAV prep |
0.5L-scale research AAV prep |
Internal
$1,700.00
each
UT System/In Network $2,550.00 each Commercial/Industry $3,400.00 each |
|
1L-scale research AAV prep |
Internal
$2,500.00
each
UT System/In Network $3,750.00 each Commercial/Industry $5,000.00 each |
||
250ml_scale research AAV prep (CsCl method) | Inquire | ||
500L-scale GMP production lot |
Internal
$950,000.00
each
UT System/In Network $1,425,000.00 each External $1,900,000.00 each |
||
50L-scale Tox production lot |
50L Tox Lot run execlude external performed Tox QC release testing |
Inquire | |
Plasmid Ratio Study |
Internal
$7,330.00
each
UT System/In Network $11,000.00 each External $14,660.00 each |
||
2L-scale research AAV prep |
Internal
$4,400.00
each
UT System/In Network $6,600.00 each Commercial/Industry $8,800.00 each |
||
5L-scale research AAV prep |
Internal
$9,500.00
each
UT System/In Network $14,250.00 each External $19,000.00 each |
||
10L-scale research AAV prep |
Internal
$17,500.00
each
UT System/In Network $26,250.00 each External $35,000.00 each |
||
20L-scale research AAV prep |
Internal
$28,500.00
each
External $57,000.00 each UT System/In Network $42,750.00 each |
||
50L-scale research AAV prep |
Internal
$90,000.00
each
UT System/In Network $135,000.00 each External $180,000.00 each |
||
► Samples (10) | |||
Name | Description | Price | |
Biodistribution qPCR per tier 1 (1-36 samples) |
Internal
$170.00
each
|
||
Biodistribution qPCR per tier 2 (37-72 samples) |
Internal
$330.00
each
|
||
Biodistribution qPCR per tier 3 (73-96 samples) | Inquire | ||
Biodistribution qPCR setup fee (IND-enabling) |
Internal
$750.00
each
External $950.00 each |
||
Biodistribution qPCR setup fee (standard) |
Internal
$100.00
each
External $200.00 each |
||
ELISA Testing (VP) 25-32 Samples | Inquire | ||
Endotoxin |
Internal
$75.00
each
|
||
Potency Testing |
Internal
$5,000.00
each
|
||
Vg Virus Titer (AAV) by qPCR (1-15 Samples) |
Internal
$75.00
each
|
||
Vg Virus Titer (AAV) by qPCR (16-36 Samples) |
Internal
$95.00
each
|