New: Lower Price for B6/J, Effective 1 March 2021
The mission of the UTSW Transgenic Core is to create, propagate, and preserve lines of genetically engineered rodents. The Core staff generate conventional transgenic mice and rats from various inbred backgrounds using either single or multiple small transgenes and BACs. The Core has been creating genome edited rodents for ~ 6 years using the latest CRISPR/Cas9 editing tools. These efforts include creating simple KO’s, KI’s using oligo, megamer, or plasmid-based templates for homologous recombination (HR), and the creation of conditional alleles using either 2 oligo templates containing loxP motifs or a single long ssDNA template with the appropriate loxP motifs. The Core staff continue to perform gene targeting in mouse embryonic stem (ES) cells on a small scale, expansion of in-house targeted ES cell clones and consortium-derived clones, and the preparation of select targeted ES cell clones for injection into blastocysts to create chimeric mice.
The Core staff routinely provide UTSW Investigators access to a wide array of assisted reproductive techniques. These include the cryopreservation of sperm, 2-cell embryos and ovaries from select lines of mice. The frozen gametes are stored in the Core’s cryopreservation facility on the South Campus and can be distributed to Investigators world-wide. Core staff can reconstitute lines of mice from cryopreserved gametes by either thawing embryos and transferring them to surrogate females or by thawing sperm, performing in vitro fertilization followed by embryo transfers. The Core staff routinely rederive lines of mice from conventional housing to an SPF barrier status. If the need arises, the Core can rescue a line of mice that has reached reproductive senescence or propagate a line of mice so that a very large number of animals can be produced in a single day.
Services Offered
Protocols
Robert Hammer, Ph.D. | Director
robert.hammer@utsouthwestern.edu
214-645-6292
Hours | Location |
Monday to Friday: 8am-5pm |
6001 Forest Park Road |
Name | Role | Phone | Location | |
---|---|---|---|---|
John Ritter and Mylinh Nguyen |
214-645-6288
|
john.ritter@utsouthwestern.edu; mylinh.nguyen@utsouthwestern.edu
|
ND11.223
|
Price list |
► CRISPR (1) | |||
Name | Description | Price | |
---|---|---|---|
Other Strain Fee |
This fee will cover the cost of ordering a strain not routinely in use by TTC and any associated per Diem. |
Inquire | |
► Cryopreservation (1) | |||
Name | Description | Price | |
Vapor Shipper - LN2 Charge |
Fee to cover the cost of adding LN2 to a dry-shipper in preparation of gamate transfer. |
Inquire |