The mission of the UTSW Transgenic Core is to create, propagate, and preserve lines of genetically engineered rodents. The Core staff generate conventional transgenic mice and rats from various inbred backgrounds using either single or multiple small transgenes and BACs. The Core has been creating genome edited rodents for ~ 6 years using the latest CRISPR/Cas9 editing tools. These efforts include creating simple KO’s, KI’s using oligo, megamer, or plasmid-based templates for homologous recombination (HR), and the creation of conditional alleles using either 2 oligo templates containing loxP motifs or a single long ssDNA template with the appropriate loxP motifs. The Core staff continue to perform gene targeting in mouse embryonic stem (ES) cells on a small scale, expansion of in-house targeted ES cell clones and consortium-derived clones, and the preparation of select targeted ES cell clones for injection into blastocysts to create chimeric mice.
The Core staff routinely provide UTSW Investigators access to a wide array of assisted reproductive techniques. These include the cryopreservation of sperm, 2-cell embryos and ovaries from select lines of mice. The frozen gametes are stored in the Core’s cryopreservation facility on the South Campus and can be distributed to Investigators world-wide. Core staff can reconstitute lines of mice from cryopreserved gametes by either thawing embryos and transferring them to surrogate females or by thawing sperm, performing in vitro fertilization followed by embryo transfers. The Core staff routinely rederive lines of mice from conventional housing to an SPF barrier status. If the need arises, the Core can rescue a line of mice that has reached reproductive senescence or propagate a line of mice so that a very large number of animals can be produced in a single day.
Services Offered
Protocols
Robert Hammer, Ph.D. | Director
robert.hammer@utsouthwestern.edu
214-645-6292
Hours | Location |
Monday to Friday: 8am-5pm |
6001 Forest Park Road |
Name | Role | Phone | Location | |
---|---|---|---|---|
John Ritter and Mylinh Nguyen |
214-645-6288
|
john.ritter@utsouthwestern.edu; mylinh.nguyen@utsouthwestern.edu
|
ND11.223
|
Price list |
► CRISPR (17) | |||
Name | Description | Price | |
---|---|---|---|
KnockOut - INDEL/Exon loss - C57BL/6N mouse eggs / per 200 embryos |
Delivery of CRISPR reagents into C57BL/6N mouse eggs |
Inquire | |
KnockOut - INDEL/Exon loss - C57BL/6J mouse eggs / per 200 embryos |
Delivery of CRISPR reagents into C57BL/6J mouse eggs |
Internal
$3,182.00
each
UT System/In Network $3,182.00 each External $6,364.00 each Commercial/Industry $6,364.00 each |
|
KnockIn - Oligo 'less than' 200bp ssODN - C57BL/6N mouse eggs / per 300 embryos |
Delivery of CRISPR reagents into C57BL/6N mouse eggs |
Internal
$3,182.00
each
UT System/In Network $3,182.00 each External $6,364.00 each Commercial/Industry $6,364.00 each |
|
KnockIn - Oligo 'less than' 200bp ssODN - C57BL/6J mouse eggs / per 300 embryos |
Delivery of CRISPR reagents into C57BL/6J mouse eggs |
Internal
$3,182.00
each
UT System/In Network $3,182.00 each External $6,364.00 each Commercial/Industry $6,364.00 each |
|
KnockIn - Megamer 'greater than' 200bp ssODN - C57BL/6N mouse eggs / per 400 embryos |
Delivery of CRISPR reagents into C57BL/6N mouse eggs |
Internal
$3,394.00
each
UT System/In Network $3,394.00 each External $6,788.00 each Commercial/Industry $6,788.00 each |
|
KnockIn - Megamer 'greater than' 200bp ssODN - C57BL/6J mouse eggs / per 400 embryos |
Delivery of CRISPR reagents into C57BL/6J mouse eggs |
Internal
$3,394.00
each
UT System/In Network $3,394.00 each External $6,788.00 each Commercial/Industry $6,788.00 each |
|
KnockIn - Conditional - ssODN x2 - C57BL/6N mouse eggs / per 400 embryos |
Delivery of CRISPR reagents into C57BL/6N mouse eggs |
Internal
$3,394.00
each
UT System/In Network $3,394.00 each External $6,788.00 each Commercial/Industry $6,788.00 each |
|
KnockIn - Conditional - ssODN x2 - C57BL/6J mouse eggs / per 400 embryos |
Delivery of CRISPR reagents into C57BL/6J mouse eggs |
Internal
$3,394.00
each
UT System/In Network $3,394.00 each External $6,788.00 each Commercial/Industry $6,788.00 each |
|
KnockIn - Plasmid dsDNA - C57BL/6N mouse eggs / per 400 embryos |
Delivery of CRISPR reagents into C57BL/6N mouse eggs |
Internal
$3,394.00
each
UT System/In Network $3,394.00 each External $6,788.00 each Commercial/Industry $6,788.00 each |
|
KnockIn - Plasmid dsDNA - C57BL/6J mouse eggs / per 400 embryos |
Delivery of CRISPR reagents into C57BL/6J mouse eggs |
Internal
$3,394.00
each
UT System/In Network $3,394.00 each External $6,788.00 each Commercial/Industry $6,788.00 each |
|
Knock-In - HDR-Block from IDT - C57BL/6N mouse eggs / per 400 embryos |
Alt-R HDR Block from IDT is a double strand DNA template, 201 to 3000bp in length, that is used to generate a CRISPR Knock-In's. |
Internal
$3,394.00
each
UT System/In Network $3,394.00 each External $6,788.00 each Commercial/Industry $6,788.00 each |
|
Knock-In - HDR-Block from IDT - C57BL/6J mouse eggs / per 400 embryos |
Alt-R HDR Block from IDT is a double strand DNA template, 201 to 3000bp in length, that is used to generate a CRISPR Knock-In's in the C57BL/6J mouse |
Internal
$3,394.00
each
UT System/In Network $3,394.00 each External $6,788.00 each Commercial/Industry $6,788.00 each |
|
IVF Generated Embryos and CRISPR Injections |
PI should request this service when the use of an established line of mice is needed to further generate gene edited rodents using CRISPR reagents.
|
Internal
$3,394.00
each
UT System/In Network $3,394.00 each External $6,788.00 each Commercial/Industry $6,788.00 each |
|
Rescue Conditional KO - Insert missing 5' or 3' LoxP Site |
The Core will generate embryos from the PI mouse line using IVF and then inject CRISPR reagents to insert the missing LoxP site. |
Internal
$2,121.00
each
UT System/In Network $2,121.00 each External $4,242.00 each Commercial/Industry $4,242.00 each |
|
CRISPR-modified Rats - KO / per 200 embryos |
Delivery of CRISPR reagents into Rat eggs |
Internal
$10,608.00
each
UT System/In Network $10,608.00 each External $21,216.00 each Commercial/Industry $21,216.00 each |
|
CRISPR-modified Rats - KI / per 300 embryos |
Delivery of CRISPR reagents into Rat eggs |
Internal
$10,608.00
each
UT System/In Network $10,608.00 each External $21,216.00 each Commercial/Industry $21,216.00 each |
|
Other Strain Fee |
This fee will cover the cost of ordering a strain not routinely in use by TTC and any associated per Diem. |
Inquire | |
► Transgenic Rodents (6) | |||
Name | Description | Price | |
Microinjection into the mouse pronucleus - C57Bl/6NCrL (Charles River #027) |
Microinjection of purified DNA and embryo transfers of 300 pronucleus stage C57BL/6N embryos |
Internal
$3,394.00
each
UT System/In Network $3,394.00 each External $6,788.00 each Commercial/Industry $6,788.00 each |
|
Microinjection into the mouse pronucleus - C57Bl/6J (Jackson Labs #000664) |
Microinjection of purified DNA and embryo transfers of 300 pronucleus stage C57BL/6J embryos |
Internal
$3,394.00
each
UT System/In Network $3,394.00 each External $6,788.00 each Commercial/Industry $6,788.00 each |
|
Microinjection into the mouse pronucleus - B6SJLF1 (Jackson Labs #100012) |
Microinjection of purified DNA and embryo transfers of 300 pronucleus stage B6SJLF1 embryos |
Internal
$4,243.00
each
UT System/In Network $4,243.00 each External $8,486.00 each Commercial/Industry $8,486.00 each |
|
Transient Transgenic / Developmental Assay Service |
One day Injections of DNA Shipment of Recipent Females for eDay Assay C57Bl/6N strain
|
Internal
$832.00
each
UT System/In Network $832.00 each External $1,664.00 each Commercial/Industry $1,664.00 each |
|
Isolation and Purification of the Transgene Fragment from the Parent Plasmid |
Isolation of the transgene fragment from the parent plasmid and purification for microinjection |
Internal
$424.00
each
UT System/In Network $424.00 each External $848.00 each Commercial/Industry $848.00 each |
|
Propagation of Plasmid or BAC and Purification of cirDNA |
Plasmid or BAC Propagation and DNA purification for microinjections |
Internal
$838.00
each
UT System/In Network $838.00 each External $1,676.00 each Commercial/Industry $1,676.00 each |
|
► Assisted Reproductive Techniques (ART) (4) | |||
Name | Description | Price | |
Sperm recovery, in vitro fertilization, and embryo transfer / per 2 – 4 transfers |
The Core will thaw the cryopreserved sperm, perform in vitro fertilization, culture the eggs to the 2-cell stage, and transfer the embryos to pseudopregnant recipients |
Internal
$2,121.00
Line
UT System/In Network $2,121.00 Line External $4,242.00 Line Commercial/Industry $4,242.00 Line |
|
Embryo recovery and transfer / per 2 – 4 transfers |
Thaw and recover cryopreserved embryos and transfer them to pseudopregnant recipients |
Internal
$1,273.00
Line
UT System/In Network $1,273.00 Line External $2,546.00 Line Commercial/Industry $2,546.00 Line |
|
Ovary recovery and transplantation / per 2 – 4 transfers |
Fresh or cryopreserved bisected ovaries and surgically transfer the ovary to histocompatible recipients |
Internal
$2,121.00
Line
UT System/In Network $2,121.00 Line External $4,242.00 Line Commercial/Industry $4,242.00 Line |
|
Embryo Collection (only available strains) / per line |
Harvest embryo from PI mated females or strains available in the Core |
Internal
$630.00
each
UT System/In Network $630.00 each External $1,260.00 each Commercial/Industry $1,260.00 each |
|
► Cryopreservation (7) | |||
Name | Description | Price | |
Cryopreservation – Mouse Embryos |
This service will provide the PI cryopreserved for their transgenic and knock-out strains of mice at approximately 200 fertilized eggs and 35 eggs/vial. |
Internal
$1,485.00
Line
UT System/In Network $1,485.00 Line External $2,970.00 Line Commercial/Industry $2,970.00 Line |
|
Cryopreservation – Mouse Sperm |
This service will provide the PI with cryopreserve sperm from their transgenic and knock-out strains of mice 1. 10 µl/straw with an estimate of 8-10 straws per male |
Internal
$1,060.00
Line
UT System/In Network $1,060.00 Line External $2,120.00 Line Commercial/Industry $2,120.00 Line |
|
Cryopreservation of Sperm for Shipment |
The PI will provide 1 proven stud males to the Core in order to perform cryopreservation of a mouse line for the purpose of shipping to another institution. |
Internal
$500.00
each
UT System/In Network $500.00 each External $1,000.00 each Commercial/Industry $1,000.00 each |
|
Cryopreservation – Mouse Ovaries (bisected) |
This service will provide the PI with cryopreserved, bisected ovaries from their transgenic and knock-out strains of mice. 1. Both ovaries are bisected and stored as a half per vial. 2. Up to 3 females per line. |
Internal
$1,060.00
Line
UT System/In Network $1,060.00 Line External $2,120.00 Line Commercial/Industry $2,120.00 Line |
|
Cryopreservation of Rat Sperm |
This service will provide the PI cryopreserve sperm from their transgenic and knock-out strains of mice; 10 µl/straw / per 8-10 straws / male |
Internal
$1,500.00
each
UT System/In Network $1,500.00 each External $3,000.00 each Commercial/Industry $3,000.00 each |
|
Storage / per 1 year |
Annual Storage Fee - after the first year |
Internal
$212.00
Line
UT System/In Network $212.00 Line External $424.00 Line Commercial/Industry $424.00 Line |
|
Vapor Shipper - LN2 Charge |
Fee to cover the cost of adding LN2 to a dry-shipper in preparation of gamate transfer. |
Internal
$106.00
each
UT System/In Network $106.00 each External $212.00 each Commercial/Industry $212.00 each |
|
► Rederivation (1) | |||
Name | Description | Price | |
Rederivation of Mouse Line |
Rederive 2-3 litters from contaminated line into a SPF status |
Internal
$1,273.00
Line
UT System/In Network $1,273.00 Line External $2,546.00 Line Commercial/Industry $2,546.00 Line |